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ANALISIS DE LA IMPLANTACION MEDIANTE EL GENOTIPO DEL GRUPO SANGUINEO ABO, EN TRASPLANTE DE CELULAS MADRE HEMATOPOYETICAS ABO- INCOMPATIBLES

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Se verifica, mediante el empleo de técnicas de biología celular, que el análisis del genotipo ABO es de gran utilidad para detectar la implantación celular de un donante ABO-incompatible luego de TMH.
kazuo9.jpg Autor:
Kazuo Muroi,
Columnista Experto de SIIC
Artículos publicados por Kazuo Muroi,
Coautores
Koji Kishino*  Chizuru Kawano-Yamamoto**  Takuji Miyoshi***  Yoko Nakagi*  Takahiro Nagashima**  Masaki Mori, Keiya Ozawa** 
Medical Technologist. Division of Cell Transplantation and Transfusion, Jichi Medical School*
Division of Cell Transplantation and Transfusion, Jichi Medical School**
Division of Hematology, Jichi Medical School***
Recepción del artículo
10 de Marzo, 2004 		Aprobación
0 de Julio, 2004
Primera edición
25 de Febrero, 2005 		Segunda edición, ampliada y corregida
7 de Junio, 2021

ANALISIS DE LA IMPLANTACION MEDIANTE EL GENOTIPO DEL GRUPO SANGUINEO ABO, EN TRASPLANTE DE CELULAS MADRE HEMATOPOYETICAS ABO- INCOMPATIBLES

Resumen
Luego del trasplante de células madre hematopoyéticas (TMH) practicado en 12 pacientes se analizó la implantación mediante el estudio del genotipo del grupo sanguíneo ABO (ABO) en la unidad formadora de colonias eritrocitaria (UFC- E) y en las células mononucleares (CMN) de sangre periférica. En 10 pacientes tratados con regímenes mieloablativos se encontraron los genotipos ABO completos del donante en el día 21 posterior al trasplante. Al analizarse el genotipo ABO en cada paciente se observó recuperación hematopoyética autóloga y quimerismo mixto transitorio. El análisis del genotipo ABO es útil para la evaluación de la implantación luego de un TMH ABO-incompatible.

Palabras clave
Trasplante de células madre hematopoyéticas, implantación, genes del grupo sanguíneo ABO, UFC-E, biología molecular


Artículo completo
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ANALYSIS OF ENGRAFMENT USING ABO BLOOD GROUP GENOTYPING IN ABO-INCOMPATIBLE HEOMPOIETIC STEM CELL TRANSPLANTATION

Abstract
Background: The analysis of chimerism after hemopoietic stem cell transplantation (HST) is very important to evaluate sustained engraftment of donor cells. Although several methods have been developed, most of these methods identify differentiated cells such as mononuclear cells (MNCs), granulocytes and lymphocytes derived from donor cells. Since cDNAs for A transferase and B transferase were recently cloned, using PCR and clonal cell culture techniques, ABO blood group (ABO) genotypes of erythroid burst-forming units (BFU-E) can be analyzed after ABO-incompatible HST. Materials, methods and results: DNA was serially extracted from erythroid colonies and peripheral blood MNCs in 12 patients who received an ABO-incompatible hemopoietic stem cell transplant. DNA samples were amplified by PCR using specific primers for the A, B and O alleles. Flow cytometric identification of red blood cells (RBCs) was conducted using monoclonal antibodies specific for ABO antigens. Complete conversion to the donor’s ABO genotype was found on day +21 in 10 patients treated with myeloablative regimens. In contrast, recipient’s RBCs were detected until day +85. Autologous hemopoietic recovery was early detected by ABO genotypic analysis of BFU-E in one patient after HST. Another patient treated with a non-myeloablative regimen showed a transient mixed ABO genotypic chimerism of BFU-E and peripheral blood MNCs, followed by complete donor ABO genotypic chimerism in both cells. Conclusion: ABO genotypic analysis is useful for evaluating engraftment of donor cells after ABO-incompatible HST, although identifying complete donor chimerism, complete differences in ABO genes between the donor and recipient are necessary.

Key words
Hemopoietic stem cell transplantation, engraftment, chimerism, ABO blood group gene, BFU-E


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Especialidades
Principal: Hematología, Trasplantes
Relacionadas: Bioquímica, Diagnóstico por Laboratorio, Medicina Interna



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Bibliografía del artículo
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