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LA MELATONINA EVITA EL ESTRES OXIDATIVO INDUCIDO POR ACIDO OCADAICO Y PEROXIDO DE HIDROGENO EN CELULAS N1E-115

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Estos datos sugieren la importancia del estrés oxidativo en el modelo del ácido ocadaico, así como su participación en los cambios bioquímicos originados en el curso y evolución de este modelo experimental, similar a la enfermedad de Alzheimer
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Montilla lópez, pedro
Columnista Experto de SIIC

Institución:
Departamento de Bioquímica y Biología Molecular Facultad de Medicina Universidad de Córdoba Córdoba, España

Artículos publicados por Montilla lópez, pedro
Coautores
Isaac Túnez Fiñana. Doctor en Medicina y Ciru*  María del Carmen Muñoz de Agueda. Diplomada U* 
*
Recepción del artículo
27 de Abril, 2004 		Aprobación
10 de , 2004
Primera edición
1 de Diciembre, 2004 		Segunda edición, ampliada y corregida
7 de Junio, 2021

LA MELATONINA EVITA EL ESTRES OXIDATIVO INDUCIDO POR ACIDO OCADAICO Y PEROXIDO DE HIDROGENO EN CELULAS N1E-115

Resumen
El presente trabajo, realizado en células de neuroblastoma N1E-115, evalúa el estrés oxidativo inducido por ácido ocadaico, peróxido de hidrógeno y ácido ocadaico más peróxido de hidrógeno, así como el efecto protector de la melatonina. Nuestro estudio muestra que la exposición de las células al ácido ocadaico (50 nM) y peróxido de hidrógeno (200 μM) durante dos horas induce un intenso estrés oxidativo caracterizado por descenso en el contenido de GSH y reducción en la actividad celular de glutatión transferasa y catalasa. Asimismo, estas condiciones experimentales desencadenan un incremento en los productos de lipoperoxidación. El grado de estrés oxidativo inducido por el ácido ocadaico casi se duplica en presencia de peróxido de hidrógeno. Los efectos inducidos por el ácido ocadaico, el peróxido de hidrógeno y el ácido ocadaico más peróxido de hidrógeno son evitados por la presencia de melatonina (10-5 M). Estos datos sugieren la importancia del estrés oxidativo en el modelo del ácido ocadaico, así como su participación en los cambios bioquímicos originados en el curso y evolución de este modelo experimental, similar a la enfermedad de Alzheimer. Estos resultados parecen indicar el efecto antioxidante y protector de la melatonina.

Palabras clave
Células N1E-115, enfermedad de Alzheimer, ácido ocadaico, estrés oxidativo, peróxido de hidrógeno, melatonina


Artículo completo
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MELATONIN PREVENTS OXIDATIVE STRESS INDUCED BY OKADAIC ACID AND HYDROGEN PEROXIDE IN N1E-115 CELLS

Abstract
The present study with N1E-115 neuroblastoma cells evaluated the oxidative stress induced by okadaic acid, hydrogen peroxide and okadacia acid plus hydrogen peroxide, as well as the protective effect of melatonin. We demonstrated that exposure of cells to 50 nM acid and hydrogen peroxide (200 μM) for 2 h induced an intense oxidative stress characterized by a decrease of the GSH content and a reduction in cellular glutathione transferase and catalase activity. Likewise, these experimental conditions prompted an increase in lipid peroxidation products. The degree of oxidative stress induced by okadaic acid was almost duplicated in the presence of hydrogen peroxide. The effects induced by okadaic acid, hydrogen peroxide and okadaic acid plus hydrogen peroxide are prevented by melatonin (10-5 M). These data indicate the great importance of oxidative stress in the okadaic acid model, as well as its participation in the biochemical changes originated in the course and development of this experimental model, similar to Alzheimer's disease. These results seem to indicate the antioxidative and protective effect of melatonin.

Key words
N1E-115 cells, Alzheimer's disease, okadaic acid, oxidative stress, hydrogen peroxide, melatonin

Clasificación en siicsalud
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Especialidades
Principal: Bioquímica
Relacionadas: Farmacología, Medicina Interna



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Especialidad principal:
Bioquímica


Relacionadas:
Farmacología
Medicina Interna
 
 
 
 
 
 
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