Manuel Monteagudo Jiménez *

Autor invitado por SIIC

UTILIDAD DE LOS NIVELES DE PLAQUETAS RETICULADAS EN EL DIAGNOSTICO DE PACIENTES TROMBOCITOPENICOS

La determinación de los niveles de plaquetas reticuladas mediante citometría de flujo es un marcador periférico indirecto de la actividad megacariocítica medular y podría ser una buena prueba de tamizaje en pacientes trombocitopénicos, como el de reticulocitos lo es en la anemia. Un porcentaje elevado de plaquetas reticuladas, superior al 11%, junto con la evaluación clínica permitiría el diagnóstico de trombocitopenias regenerativas.

*Manuel Monteagudo Jiménez
describe para SIIC los aspectos relevantes de su trabajo
RETICULATED PLATELETS AS A SCREENING TEST TO IDENTIFY THROMBOCYTOPENIA AETIOLOGY
Institución principal de la investigación
*Hospital de Sabadell, Institut Universitari Parc Tauli?uab, Sabadell, Barcelona,, Sabadell, España
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
Ingram M, Coopersmith A. Reticulated platelets following acute blood loss. Br J Haematol 17(3):225-9, 1969.
Kienast J, Schmitz G. Flow cytometric analysis of thiazole orange uptake by platelets: a diagnostic aid in the evaluation of thrombocytopenic disorders. Blood 75(1):116-21, 1990.
Ault KA, Rinder HM, Mitchell J, Carmody MB, Vary CP, Hillman RS. The significance of platelets with increased RNA content (reticulated platelets). A measure of the rate of thrombopoiesis. Am J Clin Pathol 98(6):637-46, 1992.
Koike Y, Yoneyama A, Shirai J, Ishida T, Shoda E, Miyazaki K et al. Evaluation of thrombopoiesis in thrombocytopenic disorders by simultaneous measurement of reticulated platelets of whole blood and serum thrombopoietin concentrations. Thromb Haemost 79(6):1106-10, 1998.
Robinson MS, MacKie IJ, Machin SJ, Harrison P. Two colour analysis of reticulated platelets. Clin Lab Haematol 22(4):211-3, 2000.
Matic GB, Chapman ES, Zaiss M, Rothe G, Schmitz G. Whole blood analysis of reticulated platelets: improvements of detection and assay stability. Cytometry 34(5):229-34, 1998.
Robinson MS, MacKie IJ, Machin SJ, Harrison P. Two colour analysis of reticulated platelets. Clin Lab Haematol 22(4): 211-3, 2000.
George JN, Woolf SH, Raskob GE, Wasser JS, Aledort LM, Ballem PJ et al. Idiopathic thrombocytopenic purpura: a practice guideline developed by explicit methods for the American Society of Hematology. Blood 88(1):3-40, 1996.
Ryningen A, Apelseth T, Hausken T, Bruserud O. Reticulated platelets are increased in chronic myeloproliferative disorders, pure erythrocytosis, reactive thrombocytosis and prior to hematopoietic reconstitution after intensive chemotherapy. Platelets 17(5):296-302, 2006.
Rinder HM, Munz UJ, Ault KA, Bonan JL, Smith BR. Reticulated platelets in the evaluation of thrombopoietic disorders. Arch Pathol Lab Med 117(6):606-10, 1993.
Saxon BR, Mody M, Blanchette VS, Freedman J. Reticulated platelet counts in the assessment of thrombocytopenic disorders. Acta Paediatr Suppl 424:65-70, 1998.
Saxon BR, Blanchette VS, Butchart S, Lim-Yin J, Poon AO. Reticulated platelet counts in the diagnosis of acute immune thrombocytopenic purpura. J Pediatr Hematol Oncol 20(1):44-8, 1998.
Catani L, Vianelli N, Luatti S, Rizzi S, Castellani S, Valdre L et al. Characterization of autotransplant-related thrombocytopenia by evaluation of glycocalicin and reticulated platelets. Bone Marrow Transplant 24(11):1191-4, 1999.
Jimenez MM, Guedan AM, Martin ML, Campos SJ, Martinez RI, Vilella TC. Measurement of reticulated platelets by simple flow cytometry: An indirect thrombocytopoietic marker. Eur J Intern Med 17(8):541-4, 2006.
Kuwana M, Okazaki Y, Satoh T, Asahi A, Kajihara M, Ikeda Y. Initial laboratory findings useful for predicting the diagnosis of idiopathic thrombocytopenic purpura. Am J Med 118(9):1026-33, 2005.
Wang C, Smith BR, Ault KA, Rinder HM. Reticulated platelets predict platelet count recovery following chemotherapy. Transfusion 42(3):368-74, 2002.
Chaoui D, Chakroun T, Robert F, Rio B, Belhocine R, Legrand O et al. Reticulated platelets: a reliable measure to reduce prophylactic platelet transfusions after intensive chemotherapy. Transfusion 45(5):766-72, 2005.
Salvagno GL, Montagnana M, Degan M, Marradi PL, Ricetti MM, Riolfi P et al. Evaluation of platelet turnover by flow cytometry. Platelets 17(3):170-7, 2006.
Harrison P, Robinson MS, MacKie IJ, Machin SJ. Reticulated platelets. Platelets 8(6):379-84, 1997.
Robinson MS, MacKie IJ, Khair K, Liesner R, Goodall AH, Savidge GF et al. Flow cytometric analysis of reticulated platelets: evidence for a large proportion of non-specific labelling of dense granules by fluorescent dyes. Br J Haematol 100(2):351-7, 1998.
Robinson M, Machin S, Mackie I, Harrison P. In vivo biotinylation studies: specificity of labelling of reticulated platelets by thiazole orange and mepacrine. Br J Haematol 108(4):859-64, 2000.
Watanabe K, Takeuchi K, Kawai Y, Ikeda Y, Kubota F, Nakamoto H. Automated measurement of reticulated platelets in estimating thrombopoiesis. Eur J Haematol 54(3):163-71, 1995.
Abe Y, Wada H, Sakakura M, Nishioka J, Tomatsu H, Hamaguchi Y et al. Usefulness of fully automated measurement of reticulated platelets using whole blood. Clin Appl Thromb Hemost 11(3):263-70, 2005.
Sakakura M, Wada H, Abe Y, Nishioka J, Tomatsu H, Hamaguchi Y et al. Usefulness of measurement of reticulated platelets for diagnosis of idiopathic thrombocytopenic purpura. Clin Appl Thromb Hemost 11(3):253-61, 2005.
Briggs C, Kunka S, Hart D, Oguni S, Machin SJ. Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia. Br J Haematol 126(1):93-9, 2004.
Briggs C, Hart D, Kunka S, Oguni S, Machin SJ. Immature platelet fraction measurement: a future guide to platelet transfusion requirement after haematopoietic stem cell transplantation. Transfus Med 16(2):101-9, 2006.
Kickler TS, Oguni S, Borowitz MJ. A clinical evaluation of high fluorescent platelet fraction percentage in thrombocytopenia. Am J Clin Pathol 125(2):282-7, 2006.
Abe Y, Wada H, Tomatsu H, Sakaguchi A, Nishioka J, Yabu Y et al. A simple Technique to determine thrombopoiesis level using immature platelet fraction (IPF). Thromb Res 118(4):463-9, 2006.