María-José Escribá *

Autora invitada por SIIC

El presente estudio analiza 69 pacientes que tras una transferencia electiva infructuosa, solicitaron transferencia de sus blastocistos crioconservados

LA VITRIFICACION DE BLASTOCISTOS HUMANOS, GENETICAMENTE DIAGNOSTICADOS, INCREMENTA LA TASA ACUMULADA DE GESTACION EN CURSO POR CICLO, EMPLEANDO UN SISTEMA CERRADO

Los resultados técnicos, clínicos y el demostrado incremento significativo en la tasa acumulada de gestación permiten plantear la vitrificación de preembriones, biopsiados y diagnosticados, en estadio de blastocistos en vez de preembriones biopsiados en estadios más tempranos (día 3 de desarrollo) como estrategia a considerar en los programas de DGP.

*María-José Escribá
describe para SIIC los aspectos relevantes de su trabajo
VITRIFICATION OF PREIMPLANTATION GENETICALLY DIAGNOSED HUMAN BLASTOCYSTS AND ITS CONTRIBUTION TO THE CUMULATIVE ONGOING PREGNANCY RATE PER CYCLE BY USING A CLOSED DEVICE
Institución principal de la investigación
*Instituto Valenciano de Infertilidad. Ivi Valencia, Valencia, España
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
Munné S. Preimplantation genetic diagnosis of numerical and structural chromosome abnormalities. Reprod Biomed Online 4:183-96, 2002.
Caglar GS, Asimakopoulos B, Nikolettos N, Diedrich K, Al-Hasani S. Preimplantation genetic diagnosis for aneuploidy screening in repeated implantation failure. Reprod Biomed Online 10:381-8, 2005.
Rubio C, Pehlivan T, Rodrigo L, Simon C, Remohi J, Pellicer A. Embryo aneuploidy screening for unexplained recurrent miscarriage: a minireview. Am J Reprod Immunol 53:159-65, 2005.
Joris H, Van den Abbeel E, Vos AD, Van Steirteghem A. Reduced survival after human embryo biopsy and subsequent cryopreservation. Hum Reprod 14:2833-7, 1999.
Magli MC, Gianaroli L, Fortini D, Ferraretti AP, Munne S. Impact of blastomere biopsy and cryopreservation techniques on human embryo viability. Hum Reprod 14:770-3, 1999.
Ciotti PM, Lagalla C, Ricco AS, Fabbri R, Forabosco A, Porcu E. Micromanipulation of cryopreserved embryos and cryopreservation of micromanipulated embryos in PGD. Mol Cell Endocrinol 169:63-7, 2000.
Jericho H, Wilton L, Gook DA, Edgar DH. A modified cryopreservation method increases the survival of human biopsied cleavage stage embryos. Hum Reprod 18:568-71, 2003.
Mercader A, García-Velasco JA, Escudero E, Remohi J, Pellicer A, Simon C. Clinical experience and perinatal outcome of blastocyst transfer after coculture of human embryos with human endometrial epithelial cells: a 5-year follow-up study. Fertil Steril 80:1162-8, 2003.
Veeck LL, Bodine R, Clarke RN, Berrios R, Libraro J, Moschini RM, et al. High pregnancy rates can be achieved after freezing and thawing human blastocysts. Fertil Steril 82:1418-27, 2004.
Sathananthan H, Bongso A, Ng SC, Ho J, Mok H, Ratnam S. Ultrastructure of preimplantation human embryos co-cultured with human ampullary cells. Hum Reprod 5:309-18, 1990.
Sathananthan AH, Ratnam SS, Ng SC, Tarin JJ, Gianaroli L, Trounson A. The sperm centriole: its inheritance, replication and perpetuation in early human embryos. Hum Reprod 11:345-56, 1996.
Sathananthan AH, Tarin JJ, Gianaroli L, Ng SC, Dharmawardena V, Magli MC, et al. Development of the human dispermic embryo. Hum Reprod Update 5:553-60, 1999.
Sathananthan H, Menezes J, Gunasheela S. Mechanics of human blastocyst hatching in vitro. Reprod Biomed Online 7:228-34, 2003.
Escribá MJ, Escobedo-Lucea C, Mercader A, de los Santos MJ, Pellicer A, Remohí J. Ultrastructure of preimplantational genetic diagnosis derived human blastocysts grown in a co-culture system after vitrification. Fertil Steril 86:664-71, 2006.
Ashwood-Smith MJ, Morris GW, Fowler R, Appleton TC, Ashorn R. Physical factors are involved in the destruction of embryos and oocytes during freezing and thawing procedures. Hum Reprod 3:795-802, 1988.
Magli MC, Gianaroli L, Fortini D, Ferraretti AP, Munne S. Impact of blastomere biopsy and cryopreservation techniques on human embryo viability. Hum Reprod 14:770-3, 1999.
Jones GM, Trounson AO, Gardner DK, Kausche A, Lolatgis N, Wood C. Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod 13:169-77, 1998.
Karaki RZ, Samarraie SS, Younis NA, Lahloub TM, Ibrahim MH. Blastocyst culture and transfer: a step toward improved in vitro fertilization outcome. Fertil Steril 77:114-8, 2002.
Plachot M, Belaisch-Allart J, Mayenga JM, Chouraqui A, Serkine AM, Tesquier L. Blastocyst stage transfer: the real benefits compared with early embryo transfer. Hum Reprod 15(Suppl)6:24-30, 2000.
Milki AA, Hinckley MD, Fisch JD, Dasig D, Behr B. Comparison of blastocyst transfer with day 3 embryo transfer in similar patient populations. Fertil Steril 73:126-9, 2000.
Fanchin R, Ayoubi JM, Righini C, Olivennes F, Schonauer LM, Frydman R. Uterine contractility decreases at the time of blastocyst transfers. Hum Reprod 16:1115-9, 2001.
Menezo Y, Nicollet B, Herbaut N, Andre D. Freezing cocultured human blastocysts. Fertil Steril 58:977-80, 1992.
Menezo YJ, Nicollet B, Dumont M, Hazout A, Janny L. Factors affecting human blastocyst formation in vitro and freezing at the blastocyst stage. Acta Eur Fertil 24:207-13, 1993.
Liebermann J, Tucker MJ, Graham JR, Han T, Davis A, Levy MJ. Blastocyst development after vitrification of multipronuclear zygotes using the Flexipet denuding pipette. Reprod Biomed Online 4:146-50, 2002.
Fuller B, Paynter S. Fundamentals of cryobiology in reproductive medicine. Reprod Biomed Online 9:680-91, 2004.
Kasai M, Mukaida T. Cryopreservation of animal and human embryos by vitrification. Reprod Biomed Online 9:164-70, 2004.
Liebermann J, Tucker MJ. Vitrifying and warming of human oocytes, embryos, and blastocysts: vitrification procedures as an alternative to conventional cryopreservation. Methods Mol Biol 254:345-64, 2004.
Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H. Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53-8, 1998.
Mukaida T, Nakamura S, Tomiyama T, Wada S, Kasai M, Takahashi K. Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique. Fertil Steril 76:618-20, 2001.
Bielanski A, Nadin-Davis S, Sapp T, Lutze-Wallace C. Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology 40:110-6, 2000.
Bielanski A, Bergeron H, Lau PC, Devenish J. Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiology 46:146-52, 2003.
Bielanski A. Non-transmission of bacterial and viral microbes to embryos and semen stored in the vapour phase of liquid nitrogen in dry shippers. Cryobiology 50:206-10, 2005.
Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, et al. Successful pregnancy following blastocyst vitrification: case report. Hum Reprod 15:1802-3, 2000.
Yokota Y, Sato S, Yokota M, Yokota H, Araki Y. Birth of a healthy baby following vitrification of human blastocysts. Fertil Steril 75:1027-9, 2001. Galán A, Escribá MJ, Gámiz P, Mercader A, Rubio C, Crespo J. High survival rate of human blastocysts after preimplantation genetic diagnosis and vitrification. Hum Reprod 18:141-2, 2003.
Pérez-Albalá S, De los Santos, Galán A, Zulategui J, Remohí J, Escribá MJ. Reproductive outcomes after vitrification of human blastocysts coming from three different IVF programs. Fertil Steril 84:S173, 2005.
Escribá MJ, Escobedo-Lucea C, Mercader A, de los Santos MJ, Pellicer A, Remohí J. Ultrastructure of preimplantational genetic diagnosis derived human blastocysts grown in a co-culture system after vitrification. Fertil Steril 86:664-71, 2006.
Remohí J, Ardiles G, García-Velasco JA, Gaitán P, Simón C, Pellicer A. Endometrial thickness and serum oestradiol concentrations as predictors of outcome in oocyte donation. Hum Reprod 12:2271-6, 1997.
Escribá MJ, Bellver J, Bosch E, Sánchez M, Pellicer A, Remohí J. Delaying the initiation of progesterone supplementation until the day of fertilization does not compromise cycle outcome in patients receiving donated oocytes: a randomized study. Fertil Steril 86:92-7, 2006.

Otros artículos de María-José Escribá

Escribá MJ, Martín J, Rubio C, Valbuena D, Remohí J, Pellicer A, Simón C. Heteroparental blastocyst production from microsurgically corrected tripronucleated human embryos. Fertil Steril 86(6):1601-7, 2006.
Escribá MJ, Escobedo-Lucea C, Mercader A, De los Santos MJ, Pellicer A, Remohí J. Ultrastructure of preimplantation genetic diagnosis-derived human blastocysts grown in a coculture system after vitrification. Fertil Steril 86(3):664-71, 2006.
Escribá MJ, Bellver J, Bosch E, Sánchez M, Pellicer A, Remohí J. Delaying the initiation of progesterone supplementation until the day of fertilization does not compromise cycle outcome in patients receiving donated oocytes: a randomized study. Fertil Steril 86(1):92-7, 2006.
Escribá MJ, Valbuena D, Remohí J, Pellicer A, Simón C. New techniques on embryo manipulation. J Reprod Immunol 55(1-2):149-61, 2002.
Escribá MJ, Silvestre MA, Saeed AM, García-Ximénez F. Comparison of the effect of two different handling media on rabbit zygote developmental ability. Reprod Nutr Dev 41(2):181-6, 2001.
García-Ximéne F, Escribá MJ. Viable offspring derived from cryopreserved haploid rabbit parthenotes. Theriogenology 57(4):1319-25, 2002.
Escribá MJ, García-Ximénez F. Reconstruction of the heteroparental diploid condition in rabbit zygotes by nuclear transfer. Theriogenology 55(3):771-84, 2001.
Escribá MJ, García-Ximénez F.Use of a variable electrical pulsing sequence in rabbit oocyte activation. Reprod Nutr Dev 40(3):261-9, 2000.
Escribá MJ, García-Ximénez F. Influence of sequence duration and number of electrical pulses upon rabbit oocyte activation and parthenogenetic in vitro development. Anim Reprod Sci 59(1-2):99-107, 2000.
Escribá MJ, García-Ximénez F. Electroactivation of rabbit oocytes in an hypotonic pulsing medium and parthenogenetic in vitro development without cytochalasin B-diploidizing pretreatment. Theriogenology 51(5):963-73, 1999.

Para comunicarse con María-José Escribá mencionar a SIIC como referencia:
mjescriba@ivi.es

---

Acerca del trabajo completo

LA VITRIFICACION DE BLASTOCISTOS HUMANOS, GENETICAMENTE DIAGNOSTICADOS, INCREMENTA LA TASA ACUMULADA DE GESTACION EN CURSO POR CICLO, EMPLEANDO UN SISTEMA CERRADO

Título original en castellano
VITRIFICACION DE BLASTOCISTOS HUMANOS PERTENECIENTES AL PROGRAMA DE DIAGNOTICO GENETICO PREIMPLANTACIONAL Y SU CONTRIBUCION A LA TASA DE GESTACION EN CURSO POR CICLO, EMPLEANDO UN SISTEMA CERRADO

Autor
María-José Escribá¹
1 Bióloga, Instituto Valenciano de Infertilidad. Ivi Valencia, Valencia, España, Embrióloga Senior

Acceso a la fuente original
Fertility and Sterility
http://www.fertstert.org/

El artículo se relaciona estrictamente con las especialidades de siicsalud
El artículo se conecta secundariamente con las especialidades