Mónica Hebe Vazquez-Levin *

Autor invitado por SIIC

El estudio describe, por primera vez, la expresión del transcripto y proteína Disadherina/FXYD5 en el testículo, epidídimo y espermatozoides humanos. Asimismo, reporta su localización celular y su co-localización con Cadherina Epitelial y la Na,K-ATPasa,

EXPRESIÓN DE DISADHERINA EN EL TRACTO REPRODUCTOR MASCULINO Y EN EL ESPERMATOZOIDE HUMANO

Disadherina/FXYD5 se expresa en el testículo y epidídimo y en espermatozoides del eyaculado humano. La proteína se localiza en el acrosoma y flagelo espermáticos. Su co-localización con Cadherina Epitelial (acrosoma) y la Na,K-ATPasa (flagelo) permite proponer su rol como modulador de ambas proteínas, como se ha descripto en células somáticas.

*Mónica Hebe Vazquez-Levin
describe para SIIC los aspectos relevantes de su trabajo
EXPRESSION OF DYSADHERIN IN THE HUMAN MALE REPRODUCTIVE TRACT AND IN SPERMATOZOA
Institución principal de la investigación
*Instituto de Biología y Medicina Experimental (ibyme). Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (conicet). Fundación Ibyme., Ciudad Autónoma De Buenos Aire, Argentina
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Referencias bibliográficas
1 Marín-Briggiler CI, Veiga MF, Matos ML, González-Echeverría MF, Furlong LI, Vazquez-Levin MH. Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization. (2008). Molec. Human Reprod.; 14:561-71.
Imagen del artículo seleccionada para la portada del nro de la revista. http://molehr.oxfordjournals.org/content/vol14/issue10/cover.dtl. 2-Shamraj OI, Lingrel JB. A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis. (1994). Proc Natl Acad Sci USA; 1:2952-6. 3-Wagoner K, Sanchez G, Nguyen AN, Enders GC, Blanco G. Different expression and activity of the alpha1 and alpha4 isoforms of the Na,K-ATPase during rat male germ cell ontogeny. (2005). Reproduction; 130:627-41. 4-Woo AL, James PF, Lingrel JB. Sperm motility is dependent on a unique isoform of the Na,K-ATPase. (2000). J Biol Chem; 275:20693-9. 5-Jimenez T, Sanchez G, McDermott JP, Nguyen AN, Kumar TR, Blanco G. Increased Expression of the Na,K-ATPase alpha4 Isoform Enhances Sperm Motility in Transgenic Mice. (2011). Biol Reprod; 84:153-61. 6-Ino Y, Gotoh M, Sakamoto M, Tsukagoshi K, Hirohashi S. Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis. (2002). Proc Natl Acad Sci USA; 99:365-70. 7-Fu X, Kamps MP. E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts. (1997). Mol Cell Biol; 17:1503–12. 8-Geering, K. Function of FXYD Proteins, Regulators of Na,K-ATPase. (2005). J Bioenerg Biomembr.;37:387-92. 9-Shimamura T, Yasuda J, Ino Y, Gotoh M, Tsuchiya A, Nakajima A, Sakamoto M, Kanai Y, Hirohashi S. Dysadherin expression facilitates cell motility and metastatic potential of human pancreatic cancer cells. (2004). Cancer Res; 64:6989–95. 10-Nam JS, Kang MJ, Suchar AM, Shimamura T, Kohn EA, Michalowska AM, Jordan VC, Hirohashi S, Wakefield LM. Chemokine (C-C motif) ligand 2 mediates the prometastatic effect of dysadherin in human breast cancer cells. (2006). Cancer Res; 66:7176-84. 11-Nam JS, Hirohashi S, Wakefield LM. Dysadherin: a new player in cancer progression. (2007). Cancer Lett; 255:161–69. 12-Geering K. The functional role of beta subunits in oligomeric P-type ATPases. (2001) J Bioenerg Biomembr; 33:425-38. 13-Lubarski I, Pihakaski-Maunsbach K, Karlish SJ, Maunsbach AB, Garty H. Interaction with the Na,KATPase and tissue distribution of FXYD5 (related to ion channel). (2005). J Biol Chem; 280:37717–24. 14-Lubarski I, Karlish SJ, Garty H. Structural and functional interactions between FXYD5 and the Na,KATPase. (2007). Am J Physiol Renal Physiol; 293: F1818-F1826. 15-Lubarski I, Asher C, Garty H. Modulation of cell polarization by the Na+-K+-ATPase-associated protein FXYD5 (dysadherin). (2014). Am J Physiol Cell Physiol; 306:C1080-8. 16-Ehlers MR, Chen YN, Riordan JF. The unique N-terminal sequence of testis angiotensin-converting enzyme is heavily O-glycosylated and unessential for activity or stability. (1992). Biochem Biophys Res Commun; 183:199–205.
Vazquez-Levin MH, Marín-Briggiler CI, Caballero JN, Veiga MF. Epithelial and neural cadherin expression in the mammalian reproductive tract and gametes and their participation in fertilization-related events. Aceptado 2014. Dev Biol. 2015 May 1;401(1):2-16. Epub 2015 Jan 7.

Otros artículos de Mónica Hebe Vazquez-Levin

Furlong LI, Hellman U, Krimer A, Tezón JG, Charreau EH, Vázquez Levin MH. Expression of human proacrosin in Escherichia coli and binding to zona pellucida. Biol Reprod 62:606-615, 2000.
Marí S, Rawe V, Biancotti JC, Charreau E, Brugo Olmedo S, Dain L, Vázquez Levin MH. Biochemical and molecular studies on the proacrosin/acrosin system in patients with unexplained infertility. Fertil Steril 79(Suppl.3):1676-1679, 2003.
Marín Briggiler CI, Chertihin O, Buffone M, Herr JC, Vázquez Levin MH, Visconti P. Evidence of the presence of calcium/calmodulin-dependent protein kinase IV in human sperm and its involvement in motility regulation. J Cell Sci 118:2013-22, 2005.
Furlong LI, Harris J, Vázquez Levin MH. Binding of recombinant human proacrosin/acrosin to ZP glycoproteins. I Studies with recombinant human ZPA, ZPB and ZPC. Fertil Steril 83:1780-1790, 2005.
Marín Briggiler CI, Veiga MF, Matos ML, González Echeverría MF, Furlong LI, Vázquez Levin MH. Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization. Molec Human Reprod Epub 2008 Oct 1. 14:561-71, 2008.
Veaute C, Furlong LI, Bronson R, Harris JD, Vázquez Levin MH. Antiacrosin antibodies. I. Incidence in female patients consulting for infertility and effect upon proacrosin/acrosin activities. Fertil Steril 91:1245-55, 2009.
Veaute C, Furlong LI, Cameo M, Harris JD, Vázquez Levin MH. Antiacrosin antibodies. II. Genetic immunization with human proacrosin to assess the effect of immunity towards proacrosin/acrosin upon protein activities and mouse fertility. Fertil Steril 91:1256-68, 2009.
Marín Briggiler CI, González Echeverría MF, Munuce MJ, Ghersevich S, Caille AM, Hellman U, Corrigall VM, Vázquez Levin MH. Glucose-regulated protein 78 (Grp78/BiP) is secreted by human oviduct epithelial cells and modulates sperm-zona pellucida binding. Fertil Steril 93(5):1574-84, 2010.
Marín Briggiler CI., Lapyckyj L, González Echeverría MF, Rawe VY, Alvarez Sedó C, Vázquez Levin MH. Neural cadherin is expressed in human gametes and participates in sperm-oocyte interaction events. Int J Androl 33(1):e228-39, 2010.
Caballero JN, Gervasi MG, Veiga MF, Dalvit G, Pérez Martínez S, Cética PD, Vázquez Levin MH. Epithelial cadherin is present in bovine oviduct epithelial cells and gametes, and is involved in fertilization-related events. Theriogenology 81:1189-206, 2014.
Vázquez Levin MH, Marín Briggiler CI, Caballero JN, Veiga MF. Epithelial and neural cadherin expression in the mammalian reproductive tract and gametes and their participation in fertilization-related events. Aceptado 2014. Dev Biol 401(1):2-16, 2015. Epub 2015 Jan 7.

Para comunicarse con Mónica Hebe Vazquez-Levin mencionar a SIIC como referencia:
mhvazl@gmail.com

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Acerca del trabajo completo

EXPRESIÓN DE DISADHERINA EN EL TRACTO REPRODUCTOR MASCULINO Y EN EL ESPERMATOZOIDE HUMANO

Título original en castellano
EXPRESION DE DISADHERINA EN EL TRACTO REPRODUCTOR MASCULINO Y EN EL ESPERMATOZOIDE HUMANOS

Autor
Mónica Hebe Vazquez-Levin¹
1 Doctora en Ciencias Químicas, Instituto de Biología y Medicina Experimental (ibyme). Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (conicet). Fundación Ibyme., Ciudad Autónoma De Buenos Aire, Argentina, Investigadora Principal Conicet. Directo

Acceso a la fuente original
Fertility and Sterility
http://www.fertstert.org/

ua40317