Gerardo Barroso *

Autor invitado por SIIC

ANTES DE ESTE TRABAJO NO SE CONOCIA SI LA EDAD DEL HOMBRE IMPACTABA EN LA DETERMINACION DE MARCADORES DE APOPTOSIS EN EL ESPERMATOZOIDE

IMPACTO DE LA EDAD EN LA APOPTOSIS DEL ESPERMATOZOIDE

TRADICIONALMENTE TENEMOS EL CONCEPTO DE LA FERTILIDAD MASCULINA COMO PERPETUA, SIN EMBARGO ESTE TRABAJO DEMUESTRA QUE CON LA EDAD NO SOLO DECERECE LA CALIDAD SEMINAL SINO QUE TAMBIEN SE INCREMENTAN LOS MARCADORES APOPTOTICOS.

*Gerardo Barroso
describe para SIIC los aspectos relevantes de su trabajo
THE EFFECT OF AGE ON THE EXPRESSION OF APOPTOSIS BIOMARKERS IN HUMAN SPERMATOZOA
Institución principal de la investigación
*Instituto Nacional de Perinatologia, Mexico, México
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
1. Schwartz D, Mayaux MJ. Female fecundity as
a function of age: results of artificial insemination
in 2193 nulliparous women with azoospermic
husbands. Federation CECOS. N Engl J Med
1982;306:404–6.

2. Navot D, Bergh PA, Williams MA, Garrisi GJ,
Guzman I, Sandler B, et al. Poor oocyte quality
rather than implantation failure as a cause of agerelated
decline in female fertility. Lancet 1991;337:
1375–7.

3. Munn e S, Alikani M, Tomkin G, Grifo J, Cohen J.
Embryo morphology, developmental rates, and
maternal age are correlated with chromosome
abnormalities. Fertil Steril 1995;64:382–91.

4. Vermeulen A, Kaufman JM. Ageing of the
hypothalamo-pituitary-testicular axis in men. Horm
Res 1995;43:25–8.

5. Chahal HS, Drake WM. The endocrine system and
ageing. J Pathol 2007;211:173–80.

6. Martin RH, Spriggs E, Ko E, Rademaker AW. The
relationship between paternal age, sex ratios, and
aneuploidy frequencies in human sperm, as assessed
bymulticolor FISH.AmJHumGenet 1995;57:1395–9.

7. Sartorelli EM, Mazzucatto LF, de Pina-Neto JM.
Effect of paternal age on human sperm
chromosomes. Fertil Steril 2001;76:1119–23.

8. Flanagan JM, Popendikyte V, Pozdniakovaite N,
Sobolev M, Assadzadeh A, Schumacher A, et al.
Intra- and interindividual epigenetic variation in
human germ cells. Am J Hum Genet 2006;79:67–84.

9. Slama R, Werwatz A, Boutou O, Ducot B, Spira A,
H€ardle W. Does male age affect the risk of
spontaneous abortion? An approach using
semiparametric regression. Am J Epidemiol
2003;157:815–24.

10. Aitken RJ, De Iuliis GN. Origins and consequences of
DNA damage in male germ cells. Reprod Biomed
Online 2007;14:727–33.

11. Mathieu C, Ecochard R, Bied V, Lornage J, Czyba JC.
Cumulative conception rate following intrauterine
artificial insemination with husband’s spermatozoa:
influence of husband’s age. Hum Reprod 1995;10:
1090–7.

12. Rolf C, Behre HM, Nieschlag E. Reproductive
parameters of older compared to younger men of
infertile couples. Int J Androl 1996;19:135–42.

13. Kidd SA, Eskenazi B, Wyrobek AJ. Effects of male
age on semen quality and fertility: a review of the
literature. Fertil Steril 2001;75:237–48.

14. Eskenazi B, Wyrobek AJ, Sloter E, Kidd SA,
Moore L, Young S, et al. The association of age and
semen quality in healthy men. Hum Reprod
2003;18:447–54.

15. Vagnini L, Baruffi RL, Mauri AL, Petersen CG,
Massaro FC, Pontes A, et al. The effects of male
age on sperm DNA damage in an infertile
population. Reprod Biomed Online 2007;15:514–9.

16. Span o M, Kolstad AH, Larsen SB, Cordelli E,
Leter G, Giwercman A, et al. The applicability of
the flow cytometric sperm chromatin structure assay
in epidemiological studies. Asclepios. Hum Reprod
1998;13:2495–505.

17. Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-
Boege I, Jabs EW, et al. Advancing age has
differential effects on DNA damage, chromatin
integrity, gene mutations, and aneuploidies in
sperm. Proc Natl Acad Sci U S A 2006;103:9601–6.

18. Morris ID, Ilott S, Dixon L, Brison DR. The spectrum
of DNA damage in human sperm assessed by single
cell gel electrophoresis (Comet assay) and its
relationship to fertilization and embryo
development. Hum Reprod 2002;17:990–8.

19. Aitken RJ, De Iuliis GN, McLachlan RI. Biological
and clinical significance of DNA damage in the
male germ line. Int J Androl 2009;32:46–56.

20. Gallardo E, Sim on C, Levy M, Guanes PP, Remoh ı J,
Pellicer A. Effect of age on sperm fertility potential:
oocyte donation as a model. Fertil Steril 1996;66:
260–4.

21. Paulson RJ, Milligan RC, Sokol RZ. The lack of
influence of age on male fertility. Am J Obstet
Gynecol 2001;184:818–22.

22. Bellver J, Garrido N, Remoh ı J, Pellicer A,
Meseguer M. Influence of paternal age on assisted
reproduction outcome. Reprod Biomed Online
2008;17:595–604.

23. Campos I, G omez E, Fern andez-Valencia AL,
Landeras J, Gonz alez R, Coy P, et al. Effects of
men and recipients’ age on the reproductive
outcome of an oocyte donation program. J Assist
Reprod Genet 2008;25:445–52.

24. Frattarelli JL, Miller KA, Miller BT, Elkind-
Hirsch K, Scott RT Jr. Male age negatively impacts
embryo development and reproductive outcome in
donor oocyte assisted reproductive technology
cycles. Fertil Steril 2008;90:97–103.

25. Girsh E, Katz N, Genkin L, Girtler O, Bocker J,
Bezdin S, et al. Male age influences oocyte–donor
programresults. JAssist Reprod Genet 2008;25:137–43.

26. Duran E H, Dowling-Lacey D, Bocca S, Stadtmauer
L, Oehninger S. Impact of male age on the outcome
of assisted reproductive technologies using donor
oocytes. Reprod BioMed Online 2010;20:848–56.
27. World Health Organization. WHO laboratory manual
for the examination of human semen. 5th ed.
Switerland: WHO Press, 2010.

28. Kruger TF, Acosta A, Simmons KF, Swanson RJ,
Matta JF, Oehninger S. Predictive value of abnormal
sperm morphology in in vitro fertilization. Fertil
Steril 1988;49:112–7.

29. Taylor SL,Weng SL, Fox P, Duran EH, Morshedi MS,
Oehninger S, et al. Somatic cell apoptosis markers
and pathways in human ejaculated sperm: potential
utility as indicators of sperm quality. Mol Hum
Reprod 2004;10:825–34.

30. Weng SL, Taylor SL, Morshedi M, Schuffner A,
Duran EH, Beebe S, et al. Caspase activity and
apoptotic markers in ejaculated human sperm. Mol
Hum Reprod 2002;8:984–91.
31. Thornberry NA, Lazebnik Y. Caspases: enemies
within. Science 1998;281:1312–6.
32. Nagata S. Apoptotic DNA fragmentation. Exp Cell
Res 2000;256:12–8.

33. Martin SJ, Reutelingsperger CP, McGahon AJ,
Rader JA, van Schie RC, LaFace DM, et al. Early
redistribution of plasma membrane phosphatidylserine
is a general feature of apoptosis regardless of the
initiating stimulus: inhibition by overexpression of
Bcl-2 and Abl. J Exp Med 1995;182:1545–56.

34. Fadok VA, de Cathelineau A, Daleke DL,
Henson PM, Bratton DL. Loss of phospholipid
asymmetry and surface exposure of
phosphatidylserine is required for phagocytosis of
apoptotic cells by macrophages and fibroblasts. J
Biol Chem 2001;276:1071–7.

35. Oehninger S, Morshedi M, Weng SL, Taylor S,
Duran H, Beebe S. Presence and significance of
somatic cell apoptosis markers in human ejaculated
spermatozoa. Reprod Biomed Online 2003;7:469–76.

36. Grunewald S, Sharma R, Paasch U, Glander HJ,
Agarwal A. Impact of caspase activation in human
spermatozoa. Microsc Res Tech 2009;72:878–88.

37. Paasch U, Grunewald S, Fitzl G, Glander HJ.
Deterioration of plasma membrane is associated
with activated caspases in human spermatozoa. J
Androl 2003;24:246–52.
38. Barroso G, Morshedi M, Oehninger S. Analysis of
DNA fragmentation, plasma membrane translocation
of phosphatidylserine and oxidative stress in human
spermatozoa. Hum Reprod 2000;15:1338–44.

39. Gadella BM, Harrison RA. Capacitation induces
cyclic adenosine 3’,5’-monophosphate-dependent,
but apoptosis-unrelated, exposure of aminophospholipids
at the apical head plasma membrane of boar
sperm cells. Biol Reprod 2002;67:340–50.

40. Schuffner A, Morshedi M, Oehninger S.
Cryopreservation of fractionated, highly motile
human spermatozoa: the effect of membrane
phosphatidylserine externalization and lipid
peroxidation. Hum Reprod 2001;16:2148–53.

41. Schuffner A, Morshedi M, Vaamonde D, Duran EH,
Oehninger S. Effect of different incubation
conditions on phosphatidylserine externalization
and motion parameters of purified fractions of
highly motile human spermatozoa. J Androl
2002;23:194–201.

42. Barroso G, Taylor S, Morshedi M, Manzur F,
Gavi~no F, Oehninger S. Mitochondrial membrane
potential integrity and plasma membrane
translocation of phosphatidylserine as early
apoptotic markers: a comparison of two different
sperm subpopulations. Fertil Steril 2006;85:149–54.

43. Koppers AJ, De Iuliis GN, Finnie JM,
McLaughlin EA, Aitken RJ. Significance of
mitochondrial reactive oxygen species in the
generation of oxidative stress in spermatozoa. J Clin
Endocrinol Metab 2008;93:3199–207.

44. Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P,
Jennings Z, et al. Relative impact of oxidative stress
on the functional competence and genomic integrity
of human spermatozoa. Biol Reprod 1998;59:
1037–46.

45. Alvarez JG, Lewis S. Sperm chromatin structure
assay parameters measured after density gradient
centrifugation are not predictive of the outcome of
ART. Hum Reprod 2008;23:1235–6.

46. Kemal Duru N, Morshedi M, Oehninger S. Effects of
hydrogen peroxide on DNA and plasma membrane
integrity of human spermatozoa. Fertil Steril
2000;74:1200–7.

47. Singh NP, Muller CH, Berger RE. Effects of age on
DNA double-strand breaks and apoptosis in human
sperm. Fertil Steril 2003;80:1420–30.

48. Sati L, Ovari L, Bennett D, Simon SD, Demir R,
Huszar G. Double probing of human spermatozoa
for persistent histones, surplus cytoplasm, apoptosis
and DNA fragmentation. Reprod Biomed Online
2008;16:570–9.

49. Sakkas D, Moffatt O, Manicardi GC, Mariethoz E,
Tarozzi N, Bizzaro D. Nature of DNA damage in
ejaculated human spermatozoa and the possible
involvement of apoptosis. Biol Reprod 2002;66:
1061–7.

50. Mahfouz RZ, Sharma RK, Said TM, Erenpreiss J,
Agarwal A. Association of sperm apoptosis and
DNA ploidy with sperm chromatin quality in human
spermatozoa. Fertil Steril 2009;91:1110–8.

51. Avenda~no C, Franchi A, Duran H, Oehninger S.
DNA fragmentation of normal spermatozoa
negatively impacts embryo quality and
intracytoplasmic sperm injection outcome. Fertil
Steril 2009;91:1077–84.

52. Barroso G, Valdespin C, Vega E, Kershenovich R,
Avila R, Avenda~no C, et al. Developmental sperm
contributions: fertilization and beyond. Fertil Steril
2009;92:835–48.
2614

Otros artículos de Gerardo Barroso

G. Barroso, M. Mordhedi, S. Oehninger. Detection and quantification of early and late stages of apoptosis in human semen: analysis of sperm fractions with high and low motility and relationship with de production of reactive oxygen species (ROS)Fertil Steril 1999;72:3 Suppl 1;S180

G. Barroso, S. Oehninger The current status of male infertility diagnosis and treatment in ICSI Era Biomedicine 1999;2(3):141


G. Barroso, M. Barrionuevo, P. Rao, Ll. Graham, D. Danforth, S. Huey, A. Abuhamad, S. Oehninger. Vascular endothelial growth factor, nitric oxide, and leptine follicular levels correlate negatively with embryo quality in IVF patients Fertil Steril 1999;72(6):1024-6


Gerardo Barroso, Mahmood Morshedi, Sergio Oehninger. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa Hum. Reprod. 2000;15 (6); 1338-1344


G. Barroso, A. Kably, E. Carballo, S. Karchmer, E. Castelazo, S. Oehninger. Mitochondrial Transmembrane potential and plasma translocation of phosphatidylserine (PS) as early apoptosis markers: analysis of fractionated human spermatozoa ASRM ( abstract). Fertil Steril 2001;76(3) Suppl 1,S154

Barroso G, Oehninger S, Monzo A, Kolm P, Gibbons WE, Muasher SJ. High FSH:LH Ratio and Low LH Levels in Basal Cycle Day 3: Impact on Follicular Development ant IVF Outcome J. Assist. Reprod. Genet. 2001;18;(9):499-505


G. Barroso, H. Félix, F García-León, V. Cruz, E. Ibarrola, S. Oehninger. Preliminary experience comparing the efficiency of inhibitors of aromatase (Letrozole) and clomiphene citrate (Serophene) in controlled ovarian stimulation (COH) in a prospective clinical trial. Fértil-Steril 2004, S33


Gerardo Barroso, M.D., M.S. Steve Taylor, Ph.D. Mahmood Morshedi, Ph.D. Félix Manzur, M.D., Fernando Gaviño, M.D. and Sergio Oehninger, M.D., Ph. D. Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: a comparison of two different sperm subpopulations Fertil .Steril. 2006 Jan;85(1):149-54

Barroso G, Menocal G, Felix H, Rojas-Ruiz JC, Arslan M, Oehninger S. Comparison of the efficacy of the aromatase inhibitor letrozole and clomiphene citrate as adjuvants to recombinant follicle-stimulating hormone in controlled ovarian hyperstimulation: a prospective, randomized, blinded clinical trial. Fertil Steril. 2006 Nov;86(5):1428-31.


Barroso G, Valdespin C, Vega E, Kershenovich R, Avila R, Avendaño C, Oehninger S.
Developmental sperm contributions: fertilization and beyond. Fertil Steril. 2009 92(3):835-48.

Para comunicarse con Gerardo Barroso mencionar a SIIC como referencia:
alinnecolin@gmail.com

---

Acerca del trabajo completo

IMPACTO DE LA EDAD EN LA APOPTOSIS DEL ESPERMATOZOIDE

Título original en castellano
IMPACTO DE LA EDAD EN LA EXPRESION DE MARCADORES DE APOPTOSIS EN EL ESPERMATOZOIDE HUMANO

Autores
Gerardo Barroso¹, Alinne Colin², Nardhy Gómez-López³
1 M.s.c, Instituto Nacional de Perinatologia, Mexico, México, Investigador
2 M.sc., Nascere, Médico
3 Ph.d., Instituto Nacional de Perinatología, Investigación

Acceso a la fuente original
Fertility and Sterility
http://www.fertstert.org/

El artículo se relaciona estrictamente con las especialidades de siicsalud
El artículo se conecta secundariamente con las especialidades