Expertos Invitados





EFEITO DA ALOPREGNANOLONA SOBRE O DESENVOLVIMENTO DA TOLERANCIA RAPIDA AO EFEITO ANSIOLITICO DO ETANOL

(especial para SIIC © Derechos reservados)
barbosa9.jpg Autor:
Adriana Dias elpo barbosa
Columnista Experto de SIIC

Institución:
Universidade do Vale do Itajaí (UNIVALI)

Artículos publicados por Adriana Dias elpo barbosa
Coautores
Alice Figueiredo Kikko* Paula Karina Vitor Koerich* 
Estudante do Curso de Medicina, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brasil*
Recepción del artículo
4 de Enero, 2008 		Aprobación
31 de Enero, 2008
Primera edición
30 de Junio, 2008 		Segunda edición, ampliada y corregida
7 de Junio, 2021

EFEITO DA ALOPREGNANOLONA SOBRE O DESENVOLVIMENTO DA TOLERANCIA RAPIDA AO EFEITO ANSIOLITICO DO ETANOL

Resumen
Estudos prévios têm mostrado que o neuroesteróide isopregnanolona bloqueou o desenvolvimento da tolerância rápida ao efeito ansiolítico do etanol. O objetivo do presente estudo foi investigar a influência da alopregnanolona sobre o desenvolvimento da tolerância rápida ao efeito ansiolítico do etanol em camundongos. No primeiro experimento, o objetivo foi investigar o efeito da alopregnanolona (0.05, 0.10 ou 0.20 mg/kg) sobre a tolerância rápida ao etanol (1.5 g/kg). No segundo experimento, o efeito do flumazenil (2.0 mg/kg) sobre a influência da alopregnanolona na tolerância rápida ao etanol foi investigado. Os resultados mostram que o tratamento prévio com alopregnanolona interferiu no desenvolvimento da tolerância rápida ao efeito ansiolítico etanol. Além disso, o tratamento prévio com flumazenil não interferiu na ação inibitória da alopregnanolona sobre o desenvolvimento da tolerância rápida ao etanol.

Palabras clave
álcool, alopregnanolona, tolerância, ansiolítico, flumazenil


Artículo completo
(castellano)
Extensión:  +/-5.9 páginas impresas en papel A4
Exclusivo para suscriptores/assinantes

Effect of Allopregnanolone on Development of Rapid Tolerance to the Anxiolytic Effect of Ethanol

Abstract
It has been shown that the neurosteroid isopregnanolone blocks the development of rapid tolerance to the anxiolytic effect of ethanol. The aim of the present study was to investigate the influence of allopregnanolone on the development of rapid tolerance to the anxiolytic effect of ethanol in mice. In the first experiment, male Swiss mice were pretreated with allopregnanolone (0.05, 0.10 or 0.20 mg/kg) 30 min before administration of ethanol (1.5 g/kg). Twenty-four hours later, all animals were tested using the plus-maze apparatus. In the second experiment, the effect of pretreatment of mice with flumazenil (2.0 mg/kg) 15 min before the administration of allopregnanolone on rapid tolerance to the anxiolytic effect of ethanol was studied. The results show that pretreatment with allopregnanolone interfered with the development of rapid tolerance to the anxiolytic effect to ethanol. Furthermore, the pretreatment with flumazenil did not block the effect of allopregnanolone on the development of rapid tolerance to ethanol.

Key words
alcohol, allopregnanolone, tolerance, anxiolytic, flumazenil

Clasificación en siicsalud
Artículos originales > Expertos de Iberoamérica >
página   www.siicsalud.com/des/expertocompleto.php/

Especialidades
Principal: Farmacología
Relacionadas: Bioquímica, Medicina Interna, Neurología, Salud Mental, Toxicología



Comprar este artículo
Extensión: 5.9 páginas impresas en papel A4

file05.gif (1491 bytes) Artículos seleccionados para su compra



Enviar correspondencia a:
Adriana Dias Elpo Barbosa, Universidade do Vale do Itajaí Centro de Ciências da Saúde Coordenadoria de Medicina, 89302-202, Rua Uruguai, 458, Itajaí, Brasil
Patrocinio y reconocimiento:
La Dra. Adriana Dias Elpo Barbosa certifica que "agimos de acordo as normas da instituicao apropriada ou do organismo nacional pertinente para o uso de animais de laboratório".
Bibliografía del artículo
1. Kalant H. Research on tolerance: What can we learn from history? Alcohol Clin Exp Res 22:67-76, 1998.
2. Chandler JL, Harris RA, Crews TF. Ethanol tolerance and synaptic plasticity. Trends Pharmacol Sci 19:491-95, 1998.
3. Crabbe JC, Rigter H, Vijlen J, Strijbos C. Rapid development of tolerance to the hypothermic effect of ethanol in mice. J Pharmacol Exper Ther 208:128-33, 1979.
4. Barbosa ADE, Morato GS. Influence of neurosteroids on the development of rapid tolerance to ethanol in mice. Eur J Pharmacol 431:179-88, 2001.
5. Barreto PS, Lemos T, Morato GS. NMDA-receptor antagonists block the development of rapid tolerance to ethanol in mice. Addict Biol 3:55-64, 1998.
6. Rustay NR, Crabbe JC. Genetic analysis of rapid tolerance to ethanol's incoordinating effects in mice: inbred strains and artificial selection. Behav Genet 34(4):441-51, 2004.
7. Chan AWK, Schanley DL, Aleo MDE, Leong FW. Cross-tolerance between ethanol and chlordiazepoxide. Alcohol 2:209-13, 1985.
8. Khanna JM, Kalant H, Weiner J, Chau A. Rapid tolerance and cross tolerance as predictors of chronic tolerance and cross-tolerance. Pharmacol Biochem Behav 41:355-60, 1992.
9. Bowen CA, Purdy RH, Grant KA. Ethanol-like discriminative stimulus effects of endogenous neuroactive steroids: effect of ethanol training dose and dosing procedure. J Pharmacol Exp Ther 289:405-11, 1999.
10. Vanover KE, Suruki M, Robledo S, e col. Positive allosteric modulators of the GABAA receptor: differential interaction of benzodiazepines and neuroactive steroids with ethanol. Psychopharmacology 141:77-82, 1999.
11. Mihic SJ. Acute effects of ethanol on GABA-A and glycine receptor function. Neurochemistry International 35:115-23, 1999.
12. Pandey SC. Neuronal signaling systems and ethanol dependence. Mol Neurobiol 17:1-15, 1998.
13. Snell LD, Claffey DJ, Ruth JA, e col. Novel structure having antagonist actions at both the glycine site of the N-methyl-D-aspartate receptor and neuronal voltage-sensitive sodium channels: biochemical, electrophysiological, and behavioral characterization. J Pharmacol Exp Ther 292:215-27, 2000.
14. Venkov CD, Myers PR, Tanner MA, Su M, Vaughan DE. Ethanol increases endothelial nitric oxide production through modulation of nitric oxide synthase expression. Thromb Haemost 81:638-42, 1999.
15. Allan AM, Mayes CG, Draski LJ. Gamma-aminobutyric acid-actived chloride channels in rats selectively bred for differential acute sensitivity to alcohol. Alcohol Clin Exp Res 15(2):212-18, 1991.
16. Vandergriff JL, Mathews DB, Best PJ, Simson PE. Effects of ethanol and diazepam on spatial and non-spatial tasks in rats on an 8-arm maze. Alcohol Clin Exp Res 19:64, 1995.
17. White AM, Simson PE, Best PJ. Comparison between the effects of ethanol and diazepam on spatial working memory in the rat. Psychopharmacology 133:256-61, 1997.
18. Devaud LL, Smith FD, Grayson DR, Morrow AL. Chronic ethanol consumption differentially alters the expression of -aminobutyric acid receptor subunit mRNAs in rat cerebral cortex: competitive, quantitative reverse transcriptase-polymerase chain reaction analysis. Mol Pharmacol 48:861-68, 1995.
19. Devaud LL, Fritschy JM, Sieghart W, Morrow AL. Bi-directional alterations of GABA-A receptors subunit peptide levels in rat cortex during chronic ethanol consumption and withdrawal. J Neurochem 69:126-30, 1997.
20. Baulieu EE. Neurosteroids: of the nervous system, by the nervous system, for the nervous system. Rec Prog Res 52:1-32, 1997.
21. Compagnone NA, Mellon SH. Neurosteroids: Biosynthesis and function of these novel neuromodulators. Frontiers in Neuroendocrinology 21:1-56, 2000.
22. Bowen CA, Purdy RH, Grant HA. An investigation of endogenous neuroactive steroid-induced modulation of ethanol's discriminative stimulus effects. Behav Pharmacol 10(3):297-311, 1999.
23. Lambertt JJ, Belelli D, Hill-Venning C, Peters JA. Neurosteroids and GABA-A receptor function. Trends Pharmacol Sci 16:295-303, 1995.
24. Leœkiewicz M, Budziszewska B, Jaworska-Feil L, Kubera M, Basta-Kaim A, Lasoñ W. Inhibitory effect of some neuroactive steroids on cocaine-induced kindling in mice. Pol J Pharmacol 55:1131-36, 2003.
25. Finn DA, Roberts AJ, Lotrich F, Gallaher EJ. Genetic differences in behavioral sensitivity to a neuroactive steroid. J Pharmacol Exp Ther 280:820-828, 1997.
26. Gasior M, Carter RB, Witkin JM. Neuroactive steroids: potential therapeutic use in neurological and psychiatric disorders. Trends Pharmacol Sci 20:107-112, 1999.
27. De Brito Fature C, Teixeira-Silva F, Leite JR. The anxiolytic effect of pregnancy in rats is reversed by finasteride. Pharmacol Biochem Behavior 85(3):569-74, 2006.
28. Lambert JJ, Belelli D, Peden DR, Vardy AW, Peters JA. Neurosteroid modulation of GABAA receptors. Prog Neurobiol 71:67-80, 2003.
29. Concas A, Mostallino MC, Porcu P, e col. Role of brain allopregnanolone in the plasticity of gamma-aminobutyric acid type A receptor in rat brain during pregnancy and after delivery. Proc Natl Acad Sci USA 95:13284-13289, 1998.
30. Corpechot C, Collins BE, Carey MP, Tsouros A, Robel P, Fry JP. Brain neurosteroids during the mouse estrous cycle. Brain Res 766:276-280, 1997.
31. Genazzani AD, Luisi M, Malavasi B, e col. Pulsatile secretory characteristics of allopregnanolone, a neuroactive steroid, during the menstrual cycle and in amenorrheic subjects. Eur J Endocrinol 146:347-356, 2002.
32. Herbison AE. Physiological roles for the neurosteroid allopregnanolone in the modulation of brain function during pregnancy and parturition. Prog Brain Res 133:39-47, 2001.
33. Palumbo MA, Salvestroni C, Gallo R, e col. Allopregnanolone concentration in hippocampus of prepubertal rats and female rats throughout estrous cycle. J Endocrinol Invest 18:853-856, 1995.
34. Barbosa ADE, Morato GS. Effect of epipregnanolone and pregnenolone sulfate an chronic tolerance to ethanol. Pharmacol Biochem Behav 67:459-64, 2000.
35. Barbosa ADE, Morato GS. Pregnenolone sulfate, dehydroepiandrosterone sulfate and allotetrahydrodeoxycorticosterone affect rapid tolerance to the hypothermic effect of ethanol. Brain Res Bulletin 58:99-05, 2002.
36. Debatin, T, Barbosa, ADE. Effect of isopregnanolone on rapid tolerance to the anxiolytic effect of ethanol. Rev Bras Psiquiatr 28(1):18-23, 2006.
37. Melchior CL, Allen PM. Interaction of pregnanolone and pregnenolone sulfate with ethanol and pentobarbital. Pharmacol Biochem Behav 42:605-11, 1992.
38. Barbaccia ML, Affricano D, Trabucchi M, e col. Ethanol markedly increases GABAergic neurosteroids in alcohol-preferring rats. Eur J Pharmacol 384:R1-R2, 1999.
39. O'Dell LE, Alomary AA, Vallee M, Koob GF, Fitzgerald RL, Purdy RH. Ethanol-induced increases in neuroactive steroids in the rat brain and plasma are absent in adrenalectomized and gonadectomized rats. Eur J Pharmacol 484(2-3):241-47, 2004.
40. Zaleski MJB, Nunes-Filho JR, Lemos T, Morato GS. GABAB receptors play a role in the development of tolerance to ethanol in mice. Psychopharmacology 153:415-24, 2001.
41. Crews FT, Morrow AL, Criswell H, Breese G. Effects of ethanol on ion channels. Int Rev Neurobiol 39:283-367, 1996.
42. Grobin AC, Matthews DB, Devaud LL, Morrow AL. The role of GABAA receptors in the acute and chronic effects of ethanol. Psychopharmacology 139:2-19, 1998.
43. Mihic SJ, Ye Q, Wick MJ, e col. Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature 389:385-389, 1997.
44. Morrow AL, VanDoren MJ, Penland SN, Matthews DB. The role of GABAergic neuroactive steroids in ethanol action, tolerance and dependence. Brain Res Brain Res Rev 37:98-109, 2001.
45. Martin-García E, Darbra S, Pallarès M. Intrahippocampal allopregnanolone decreases voluntary chronic alcohol consumption in non-selected rats. Proq Neuropsycopharmacology Biol Psychiatry 31(4):823-31, 2007.
46. Kumar S, Fleming RL, Morrow AL. Ethanol regulation of gamma-aminobutyric acid A receptors: genomic and nongenomic mechanisms. Pharmacol Ther 101(3):211-26, 2004.
47. Devaud LL, Alele P. Differential effects of chronic ethanol administration and withdrawal on gamma-aminobutyric acid type A and NMDA receptor subunit proteins in male and female rat brain. Alcohol Clin Exp Res 28(6):957-65, 2004.
48. Yu R, Follesa P, Ticku MK. Down-regulation of the GABA receptor subunits mRNA levels in mammalian cultured cortical neurons following chronic neurosteroid treatment. Brain Res Mol Brain Res 41:163-68, 1996.
49. Devaud LL, Morrow AL, Nguyen UTQ. Ovariectomy has minimal effects on neuroadaptations associated with ethanol dependence in female rats. Neurochem Int 37:433-42, 2000.
50. Reddy S, Kulkarni ML. Differential anxiolytic effects of neurosteroids in the mirrored chamber behavior test in mice. Brai Res 752(1-2):61-71, 1997.
51. Brot MD, Akwa Y, Purdy RH, Koob GF, Briton KT. The anxiolytic-like effects of the neurosteroid allopregnanolone: interactions with GABA(A) receptors. Eur J Pharmacol 325(1):1-7, 1997.
52. Fernández-Guasti A, Picazo O. Flumazenil blocks the anxiolytic action of allopregnanolone. Eur J Pharmacol 281(1):113-115, 1995.
 
 
 
 
 
 
 
 
 
 
 
 
Está expresamente prohibida la redistribución y la redifusión de todo o parte de los contenidos de la Sociedad Iberoamericana de Información Científica (SIIC) S.A. sin previo y expreso consentimiento de SIIC.
ua31618
Inicio/Home

Copyright siicsalud © 1997-2024 ISSN siicsalud: 1667-9008