David Tarragó *

Autor invitado por SIIC

Descripción de una nueva herramienta para identificar los serotipos neumocócicos en muestras clínicas.

PCR EN TIEMPO REAL PARA EL ESTUDIO DEL SEROTIPO NEUMOCÓCICO EN EL EMPIEMA PLEURAL PARANEUMONICO PEDIÁTRICO

La vigilancia epidemiológica de la enfermedad neumocócica invasiva requiere herramientas moleculares para el conocimiento de los serotipos y genotipos circulantes. Es imprescindible para la toma de decisiones por las autoridades sanitarias para las políticas preventivas de intervención en salud pública. Ya que el rendimiento de los cultivos microbiológicos es muy bajo, es necesaria una metodología de PCR que resuelva este problema.

*David Tarragó
describe para SIIC los aspectos relevantes de su trabajo
IDENTIFICATION OF PNEUMOCOCCAL SEROTYPES FROM CULTURE-NEGATIVE CLINICAL SPECIMENS BY NOVEL REAL-TIME PCR
Institución principal de la investigación
*Centro Nacional de Microbiología, Instituto de Salud Carlos Iii, Madrid, España
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Otros artículos escogidos

Referencias bibliográficas
1. Henrichsen J. Six newly recognized types of streptococcus pneumoniae. J Clin Microbiol 33:2759-2762, 1995.
2. Fenoll A, Jado I, Vicioso D, Casal J. Dot blot assay for the serotyping of pneumococci. J Clin Microbiol 35:764-766, 1997.
3. Thomson AH, Kumar MR, Wallis C, Balfour LI. Randomised trial of intrapleural urokinase in the treatment of childhood empyema. Thorax 57:343-347, 2002.
4. Salo P, Ortqvist A, Leinonen M. Diagnosis of bacteremic pneumococcal pneumonia by amplification of pneumolysin gene fragment in serum. J Infect Dis 171:479-482, 1995.
5. Pai R, Gertz RE, Beall B. Sequential multiplex pcr approach for determining capsular serotypes of streptococcus pneumoniae isolates. J Clin Microbiol 44:124-131, 2006.
6. Huebner RE, Dagan R, Porath N, Wasas AD, Klugman KP. Lack of utility of serotyping multiple colonies for detection of simultaneous nasopharyngeal carriage of different pneumococcal serotypes. Pediatr Infect Dis J 19:1017-1020, 2000.
7. O'Brien KL, Nohynek H. Report from a who working group: Standard method for detecting upper respiratory carriage of streptococcus pneumoniae. Pediatr Infect Dis J 22:133-140, 2003.
8. Bentley SD, Aanensen DM, Mavroidi A, et al. Genetic analysis of the capsular biosynthetic locus from all 90 pneumococcal serotypes. PLoS Genet 2:e31, 2006.
9. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 215:403-410, 1990.
10. Garcia E, Llull D, Munoz R, et al. Current trends in capsular polysaccharide biosynthesis of streptococcus pneumoniae genetic bases and medical relevance of capsular polysaccharide biosynthesis in pathogenic streptococci. Res Microbiol 151:429-435, 2000.
11. Byington CL, Korgenski K, Daly J, Ampofo K, Pavia A, Mason EO. Impact of the pneumococcal conjugate vaccine on pneumococcal parapneumonic empyema. Pediatr Infect Dis J 25:250-254, 2006.
12. Obando I, Arroyo LA, Sanchez-Tatay D, et al. Molecular epidemiology of paediatric invasive pneumococcal disease in southern spain after the introduction of heptavalent pneumococcal conjugate vaccine. Clin Microbiol Infect 13:347-348, 2007.
13. Mavroidi A, Godoy D, Aanensen DM, Robinson DA, Hollingshead SK, Spratt BG. Evolutionary genetics of the capsular locus of serogroup 6 pneumococci. J Bacteriol 186:8181-8192, 2004.
14. Schultz KD, Fan LL, Pinsky J, et al. The changing face of pleural empyemas in children: Epidemiology and management. Pediatrics 113:1735-1740, 2004.
15. Suzuki N, Seki M, Nakano Y, Kiyoura Y, Maeno M, Yamashita Y. Discrimination of streptococcus pneumoniae from viridans group streptococci by genomic subtractive hybridization. J Clin Microbiol 43:4528-4534, 2005.
16. Whatmore AM, Efstratiou A, Pickerill AP, et al. Genetic relationships between clinical isolates of streptococcus pneumoniae, streptococcus oralis, and streptococcus mitis: Characterization of "atypical" pneumococci and organisms allied to s. Mitis harboring s. Pneumoniae virulence factor-encoding genes. Infect Immun 68:1374-1382, 2000.
17. Carvalho Mda G, Tondella ML, McCaustland K, et al. Evaluation and improvement of real-time pcr assays targeting lyta, ply, and psaa genes for detection of pneumococcal DNA. J Clin Microbiol 45:2460-2466, 2007.

Otros artículos de David Tarragó

1. Fenoll A, Aguilar L, Granizo JJ, Giménez MJ, Aragoneses-Fenoll L, Mendez C, Tarragó D. Has the licensing of respiratory quinolones for adults and the 7-valent pneumococcal conjugate vaccine (PCV-7) for children had herd effects with respect to antimicrobial non-susceptibility in invasive Streptococcus pneumoniae? J Antimicrob Chemother 2008 [Epub ahead of print].
2. Soriano F, Cafini F, Aguilar L, Tarragó D, Alou L, Giménez MJ, Gracia M, Ponte MC, Leu D, Pana M, Letowska I, Fenoll A. Breakthrough in penicillin resistance? Streptococcus pneumoniae isolates with penicillin/cefotaxime MICs of 16 mg/l and their genotypic and geographical relatedness. J Antimicrob Chemother [Epub ahead of print], 2008.
3. Obando I, Muñoz-Almagro C, Arroyo LA, Tarrago D, Sanchez-Tatay D, Moreno-Perez D, Dhillon SS, Esteva C, Hernandez-Bou S, Garcia-Garcia JJ, Hausdorff WP, Brueggemann AB. Pediatric parapneumonic empyema, Spain. Emerg Infect Dis 14(9):1390-7, 2008.
4. Sánchez-Tatay D, Arroyo LA, Tarragó D, Lirola MJ, Porras A, Fenoll A, Hausdorff WP, Brueggemann AB, Obando I. Antibiotic susceptibility and molecular epidemiology of nasopharyngeal pneumococci from Spanish children. Clin Microbiol Infect 14(8):797-801, 2008.
5. Fenoll A, Aguilar L, Robledo O, Giménez MJ, Granizo JJ, Biek D, Tarragó D. In vitro activity of ceftaroline against Streptococcus pneumoniae exhibiting penicillin, amoxicillin and cefotaxime resistance. Antimicrob Agents Chemother [Epub ahead of print], 2008.
6. Fenoll A, Aguilar L, Robledo O, Giménez MJ, Granizo JJ, Biek D, Tarragó D. In vitro activity of ME1036 versus other {beta}-lactams against penicillin-resistant Streptococcus pneumoniae serotypes exhibiting higher amoxicillin than penicillin MIC. J Antimicrob Chemother [Epub ahead of print], 2008.
7. Tarragó D, Aguilar L, Jansen WT, Giménez MJ, Avellón A, Granizo JJ, Casal J. Dependence of correlations between antibody titres and opsonophagocytosis on pneumococcal serotype and patient morbidity in pre- and post-pneumococcal vaccination states. Clin Microbiol Infect 13(4):369-76, 2007.
8. Tarragó D, Aguilar L, Giménez MJ, Fenoll A, Casal J. Effects of amoxicillin subinhibitory concentrations on the cross-protection developed by pneumococcal antibodies in mouse sepsis caused by an amoxicillin-resistant serotype 6B Streptococcus pneumoniae strain. Antimicrob Agents Chemother 48(11):4144-7, 2004.
9. Casal J, Tarragó D. Immunity to Streptococcus pneumoniae: Factors affecting production and efficacy. Curr Opin Infect Dis 16(3):219-24, 2003.

Para comunicarse con David Tarragó mencionar a SIIC como referencia:
davtarrago@isciii.es

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Acerca del trabajo completo

PCR EN TIEMPO REAL PARA EL ESTUDIO DEL SEROTIPO NEUMOCÓCICO EN EL EMPIEMA PLEURAL PARANEUMONICO PEDIÁTRICO

Título original en castellano
IDENTIFICACION DE SEROTIPOS NEUMOCOCICOS MEDIANTE PCR EN TIEMPO REAL

Autor
David Tarragó¹
1 Facultativo Especialista Inves, Centro Nacional de Microbiología, Instituto de Salud Carlos Iii, Madrid, España, Investigador

Acceso a la fuente original
Clinical Microbiology and Infection
http://www.blackwellpublishing.com/journal.asp?ref=1198-743X&site=1

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