José Edgardo Escamilla Marván *

Autor invitado por SIIC

El trabajo contribuye al conocimiento de la fisiología de Bacillus cereus, su explicación genómica y sus relaciones filogenéticas

BACILLUS CEREUS Y EL SUCCINATO. TRANSPORTE, UTILIZACION Y GENOMICA

La enzima succinato:quinona reductasa (SQR) de Bacillus cereus presenta propiedades cinético-moleculares semejantes a la de B. subtilis. Sin embargo, B. cereus es incapaz de utilizar el succinato exógeno. Esto es debido a que carece de un sistema de transporte para ácidos C4-dicarboxílicos. Esta característica y otras propiedades fisiológicas apoyan la propuesta de que el ancestro común del grupo “cereus” de bacterias es un patógeno oportunista.

*José Edgardo Escamilla Marván
describe para SIIC los aspectos relevantes de su trabajo
THE SUCCINATE:MENAQUINONE REDUCTASE OF BACILLUS CEREUS-CHARACTERIZATION OF THE MEMBRANE-BOUND AND PURIFIED ENZYME
Institución principal de la investigación
*Instituto Defisiología Celular, Universidad Nacional Autónoma de México, México, México
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
Ackrell BAC. Progress in understanding structure-function relationships in respiratory chain complex II. FEBS Lett 466:1-5, 2000.
Asai K, Baik S, Kasahara Y, Moriya S, Ogasawara N. Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis. Microbiology 146:263-271, 2000.
Collins MD, Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316-354, 1981.
Escamilla JE, Barquera B, Ramírez R, García-Horsman A, Del Arenal P. Role of menaquinone in inactivation and activation of the Bacillus cereus forespore respiratory system. J Bacteriol 170:5908-5912, 1988.
Fortnagel P, Freese E. Analysis of sporulation mutants II, Mutants blocked in the citric acid cycle. J Bacteriol 95:1431-1438, 1978.
Gilmour R, Krulwich TA. Purification and characterization of the succinate dehydrogenase complex and CO-reactive b-type cytochromes from the facultative alkaliphile Bacillus firmus OF4. BB Acta 1276:57-63, 1996.
Hägerhäll C. Succinate:quinone oxidoreductases. Variations on a conserved theme. Biochim Biophys Acta 1229:356-362, 1997.
Hägerhäll C, Aasa R, Von Wachenfeldt C, Hederstedt L. Two hemes in Bacillus subtilis Succinate:menaquinone oxidoreductase (complex II). Biochemistry 31:7411-7421, 1992.
Hägerhäll C, Hederstedt L. A structural model for the membrane-integral domain of succinate: quinone oxidoreductases. FEBS Lett 389:25-31, 1996.
Hederstedt L. Cytochrome b reducible by succinate in an isolated succinate dehydrogenase-cytochrome b complex from membranes, J Bacteriology 144:933-940, 1980.
Hederstedt L. Respiration without O2. Science 284:1941-1942, 1999.
Hederstedt L. Succinate:quinone oxidoreductase in bacteria Paracoccus denitrificans and Bacillus subtilis. Biochim Biophys Acta 1553:74-83, 2002.
Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A, Chu L, Mazur M, Goltsman E, Larsen N, D'Souza M, Walunas T, Grechkin Y, Pusch G, Haselkorn R, Fonstein M, Ehrlich DS, Overbeek R, Kyrpides N. Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis. Nature 423:87-91, 2003.
Kita K, Vibat CRT, Meinhardt S, Guest JR, Gennis RB. One-step purification from Escherichia coli of complex II (succinate:ubiquinone oxidoreductase) associated with succinate-reducible cytochrome b556. J Biol Chem 264:2672-2677, 1989.
Kurokawa T, Sakamoto J. Purification and characterization of succinate: menaquinone oxidoreductase from Corynebacterium glutamicum. Arch Microbiol 183:317-324, 2005.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685, 1970.
Lancaster CR. Succinate:quinone oxidoreductases: an overview. Biochim Biophys Acta 1553:1-6, 2002.
Lin LF, Beattie DS. Purification and properties of a major cytochrome b peptide from baker's yeast. J Biol Chem 253:2412-2418, 1978.
Margulis L, Jorgensen JZ, Dolan S, Kolchinsky R, Rainey FA. The Arthromitus stage of Bacillus cereus: Intestinal symbionts of animals. Proc Natl Acad Sci USA 95:1236-1241, 1998.
Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87:206-210, 1978.
Matsson M, Ackrell BA, Cochran B, Hederstedt L. Carboxin resistance in Paracoccus denitrificans conferred by a mutation in the membrane anchor domain of succinate quinone reductase. Arch Microbiol 170:27-37, 1998.
Matsson M, Tolstoy D, Aasa R, Hederstedt L. The distal heme center in Bacillus subtilis succinate:quinone reductase is crucial for electron transfer to menaquinone. Biochemistry 39:8617-8624, 2000.
Pennoyer JD, Ohnishi T, Trumpower BL. Purification and properties of succinate-ubiquinone oxidoreductase complex from Paracoccus denitrificans. Biochim Biophys Acta 935:195-207, 1988.
Qureshi MH, Fujiwara T, Fukumori Y. Succinate:quinone oxidoreductase (complex II) containing a single heme b in facultative alkaliphilic Bacillus sp. strain YN-2000. J Bacteriol 178:3031-3036, 1996.
Schirawski J, Unden G. Menaquinone-dependent succinate dehydrogenase of bacteria catalyzes reversed electron transport driven by the proton potential. Eur J Biochem 257:210-215, 1998.
Smirnova IA, Hägerhall C, Konstantinov AA, Hederstedt L. HOQNO interaction with cytochrome b in succinate:menaquinone oxidoreducatse from Bacillus subtilis. FEBS Letters 359:23-26, 1995.
Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76:4350-4354, 1979.
Willecke K, Lange R. C4-dicarboxylate transport in Bacillus subtilis studied with 3-floro-L-erythro-malate as a substrate. J Bacteriol 117:373-378, 1974.

Otros artículos de José Edgardo Escamilla Marván

Del Arenal IP, Svlaeteorova BB, Contreras ML, Rangel P, Dávila JR, Lledías f, Escamilla JE. Haem O and a putative cytochrome bo in a mutant of Bacillus cereus impaired in the synthesis of haem A. Archives Microbiol 167:24-31, 1997.
Contreras ML, Escamilla JE, Del Arenal IP, Dávila JR, D'Mello R, Poole K. An unusual o'-type cytochrome c oxidase in a Bacillus cereus cytochrome a3 mutant has a very high affinity for oxygen. Microbiology UK 145:1563-1573, 1999.
Escamilla JE, Contreras ML, Flores M, García LM. Los sistemas respiratorios bacterianos. En Mensaje Bioquímico Vol. XXIII: Editado por Martínez F, Juárez MA, Pardo JP, Morales S. Facultad de Medicina UNAM, pp. 67-103, 1999.
Flores-Encarnación M, Contreras-Zentella M, Soto-Urzua L, Aguilar GR, Baca B, Escamilla JE. The respiratory system and the diazotrophic activity of Acetobacter diazotrophicus PAL5. J Bacteriol 181:6987-6995, 1999.
Contreras-Zentella M, Mendoza G, Membrillo-Hernández J, Escamilla JE. A novel double heme substitutions produce a functional bo3 variant of the quinol oxidase aa3 of Bacillus cereus: Purification and partial characterization. J Biol Chem 278(34):31473-31478, 2003.
Hernández-Urzúa E, Mills CE, White GP, Contreras-Zentella ML, Escamilla E, Vasudevan SG, Membrillo-Hernández J, Poole RK. Flavohemoglobin Hmp but not its individual domains confers protection from respiratory inhibition by NO in Escherichia coli. J Biol Chem 278(37):34975-34982, 2003.
Lee S, Flores-Encarnación M, Contreras Zentella M, García Flores L, Escamilla JE, Kennedy C. Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter diazotrophicus strains with mutations in cytochrome c biogenesis genes. J Bacteriol 186(16):5384-5391, 2004.
Chávez-Pacheco JL, Martínez-Yee S, Contreras ML, Gómez-Manso S, Membrillo-Hernández J, Escamilla JE. Partial bioenergetic characterization of Gluconacetobacter xylinum cells released from cellulose by a novel methodology. J Applied Microbiol 99(5):1130-1140, 2005.
Jaramillo RD, Suárez P, Barraza B, Lara P, Therán L, Escamilla JE. Eikenella corrodens: Patogénesis y aspectos clínicos. Colombia Médica 37:228 241, 2006
González B, Martínez S, Chávez JL, Lee S, Castro NA, Domínguez MA, Gómez S, Contreras ML, Kennedy C, Escamilla JE. Respiratory system of Gluconacetobacter diazotrophicus PAL-5. Evidence for a cyanide-sensitive cytochrome bb and cyanide-resistant cytochrome ba quinol oxidases. Biochimica et Biophysica Acta 1757(12):1614-1622, 2006.
Gómez-Manzo S, Contreras-Zentella M, González-Valdez A, Sosa-Torres M, Arreguín-Espinoza R, Escamilla-Marván E. The PQQ-Alcohol Dehydrogenase of Gluconacetobacter diazotrophicus. Int Jour of Food Microbiol 125(1):71-78, 2008.

Para comunicarse con José Edgardo Escamilla Marván mencionar a SIIC como referencia:
eescami@ifc.unam.mx

---

Acerca del trabajo completo

BACILLUS CEREUS Y EL SUCCINATO. TRANSPORTE, UTILIZACION Y GENOMICA

Título original en castellano
LA SUCCINATO:MENAQUINONA REDUCTASA DE BACILLUS CEREUS. CARACTERIZACION DE LA ENZIMA MEMBRANAL Y DE LA ENZIMA PURIFICADA

Autores
José Edgardo Escamilla Marván¹, Martha Lucinda Contreras Zentella², Rúben Darío Jaramillo Lanchero³
1 Doctor en Ciencias Químicas, Instituto Defisiología Celular, Universidad Nacional Autónoma de México, México, México, Investigador Titular
2 Doctora en Ciencias Biomédicas, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Técnico Académico Titular
3 Doctor en Ciencias, Universidad de Sucre, Colombia, Investigador

Acceso a la fuente original
Canadian Journal of Microbiology
http://rcm.cnrc.ca

El artículo se relaciona estrictamente con las especialidades de siicsalud
El artículo se conecta secundariamente con las especialidades