Paula Ruybal *

Autora invitada por SIIC

ESTRATEGIA PARA LA OPTIMIZACION DE LA EXPRESION DE GENES TERAPEUTICOS EN LINFOMAS T

Numerosos grupos de investigación alrededor del mundo se encuentran evaluando protocolos de terapia génica para el tratamiento de diferentes enfermedades. Entre éstas se encuentra el cáncer, que luego de las enfermedades cardiovasculares representa la segunda causa de muerte en la población mundial.

*Paula Ruybal
describe para SIIC los aspectos relevantes de su trabajo
TRANSGENE EXPRESSION ENHANCEMENT IN T-LYMPHOMA CELL LINES
Institución principal de la investigación
*Centro de Estudios Farmacológicos y Botánicos, Cefybo-conicet, Buenos Aires, Argentina, Buenos Aires, Argentina
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
1. Buttgereit P, Schmidt-Wolf IG. Gene therapy of lymphoma. J Hematother Stem Cell Res 2002; 11:457-467.
2. Pfeifer A, Verma IM. Gene therapy: promises and problems. Annu Rev Genom Hum Genet 2001; 2:177-211.
3. Sutherland LC, Williams GT. Viral promoter expression CEMC7 and Jurkat human T-lymphoid cell lines. J Immunol Methods 1997; 207:179-183.
4. Zarrin AA, Malkin L, Fong I, Luk KD, Ghose A, Berinstein NL. Comparison of CMV, RSV, SV40 viral and Vl1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochim Biophys Acta 1996; 1446:135-139.
5. Nishizuka Y. Turnover of inositol phospholipids and signal transduction. Science 1984; 225:1365-1370.
6. Karin M, Smeal T. Control of transcription factors by signal transduction pathways: the beginning of the end. Trends Biochem Sci 1992; 17:418-422.
7. Mongini C, Ruybal P, Gravisaco MJ, Croci M, Sa´nchez Lockhart M, Fabris V, et al. Characterization of the immunophenotype and the metastatic properties of a murine T-lymphoma cell line. Unexpected expression of cytoplasmatic CD4. In vitro Cell Dev Biol-Animal 2001; 37:499-504.
8. Gorer PA. Studies in antibody response of mice to tumour inoculation. Br J Cancer 1950; 4:372-379.
9. Cremaschi GA, Fisher P, Boege F. b-Adrenoceptor distribution in murine lymphoid cell lines. Immunopharmacology 1991; 22:195-206.
10. Boshart M, Weber F, Jahn G, Dorsch-Hasler K, Fleckenstein B, Schaffner W. A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 1985; 41(2):521-530.
11. Karin M, Liu Z, Zandi E. AP-1 function and regulation. Curr Opin Cell Biol 1997; 270(16):483-486.
12. Steffan NM, Bren GD, Frantz B, Tocci MJ, O’Neill EA, Paya CV. Regulation of IkB alpha phosphorylation by PKC and Ca(2+)-dependent signal transduction pathways. J Immunol 1995; 155:4685-4691.
13. Ghazal P, Lubou H, Flenckenstein B, Hennighausen L. Binding of transcription factors and creation of a large nucleoprotein complex on the human cytomegalovirus enhancer. Proc Natl Acad Sci USA 1987; 84:3658-3662.
14. Hunninghake GW, Monick MM, Liu B, Stinki MF. The promoter-regulatory region of the major immediate-early gene of human cytomegalovirus responds to T-lymphocyte stimulation and contains functional cyclic AMP response elements. J Virol 1989; 63:3026-3033.
15. Sambucetti LC, Cherrington JM, Wilkinson GWG, Mocarski ES. NFkB activation of the cytomegalovirus enhancer is mediated by a viral transactivator and by T cell stimulation. EMBO J 1989; 8:4251-4258.
16- Lo Ser P, Jennings GS, Strauss M, Sandig V. Reactivation of the previously silenced cytomegalovirus major immediate-early promoter in the mouse liver: involvement of NFkB. J Virol 1998; 72:180-190.
17. Clesham GJ, Brown H, Efstathious S, Weissberg PL. Enhancer stimulation unmasks latent gene transfer after adenovirus-mediated gene delivery to human vascular smooth muscle cells. Circ Res 1996; 79:1188-1195.
18. Wilkinson GWG, Akrigg A. Constitutive and enhanced expression from the CMV major IE promoter in a defective adenovirus vector. Nucleic Acids Res 1992; 30:2233-2239.
19. Lang D, Fickenscher H, Stamminger. Analysis of proteins binding to the proximal promoter region of the human cytomegalovirus IE-1/2 enhancer/promoter reveals both consensus and aberrant recognition sequences for transcription factors Sp1 and CREB. Nucleic Acids Res 1994; 22:2453-2459.
20. Zhang XY, Inamdar NM, Supakar PC, Wu K, Ehrlich KC, Ehrlich M. Three MDBP sites in the immediate-early enhancer-promoter region of human cytomegalovirus. Virology 1991; 182:865-869.
21. Gorelik G, Barreiro Arcos ML, Klecha AJ, Cremaschi GA. Differential expression of protein kinase C isoenzymes related to high nitric oxide synthase activity in a T lymphoma cell line. Biochim Biophys Acta 2002; 1588:179-188.

Otros artículos de Paula Ruybal

Gravisaco MJ, Mongini C, Ruybal P, Escalada A, Sánchez Lockhart M, Alvarez E, Hajos SE, Waldner C. IL-2, IL-10, IL-15 and TNF are key regulators of a murine T-cell lymphoma growth. Int J Molecular Medicine 2003, 12(4):627-632.

Barcala V, Ruybal P, García Rivello H, Waldner C, Ascione A, Mongini C. TRANCE expression in haematological B malignancies. A report in follicular lymphoma. Eur J Haematology, 2003; 70:417-419.

Mongini C, Ruybal P, Gravisaco MJ, Croci M, Sánchez Lockhart M, Fabris V, Waldner C. Characterization of the immunophenotype and the metastatic properties of a murine T-lymphoma cell line. Unexpected expression of cytoplasmatic CD4. In Vitro Cell Dev Biol Animal 2001; 37:499-504.

Mongini C, Ruybal P, García Rivello H, Mocetti E, Escalada A, Christiansen S, Argibay P. Apoptosis in gut-associated lymphoid tissue (GALT): A response to injury or a physiologic mechanism? Transplantation Proceedings 1998; 30(6):2673-2676.

Para comunicarse con Paula Ruybal mencionar a SIIC como referencia:
pruybal@cicv.inta.gov.ar

---

Acerca del trabajo completo

ESTRATEGIA PARA LA OPTIMIZACION DE LA EXPRESION DE GENES TERAPEUTICOS EN LINFOMAS T

Título original en castellano
Aumento de la expresion de un transgen en lineas celulares de derivadas de linfomas T

Autores
Paula Ruybal¹, María José Gravisaco², Virna Barcala³, Ana Escalada⁴, Graciela Cremaschi⁵, Oscar Taboga⁶, Claudia Waldner⁷, Claudia Mongini⁸
1 Lic. en Ciencias Biológicas, Instituto de Biotecnología, Cicvya, Instituto Nacional de Tecnología Agropecuaria, Becaria Posdoctoral
2 Bioquímica, Laboratorio de Inmunología Molecular y Celular, Centro de Estudios Farmacológicos, Becaria Posdoctoral
3 Bioquímica, Citomlab, Hospital Italiano de Buenos Aires
4 Bioquímica, Citomed, Buenos Aires, Argentina
5 Bioquímica, Laboratorio de Neuroinmunomodulación, Centro de Estudios Farmacológicos y Botánicos, Investigadora del Conicet
6 Lic. en Ciencias Biológicas, Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias, Investigador del Conicet
7 Bioquímica, Laboratorio de Inmunología Molecular y Celular, Centro de Estudios Farmacológicos, Investigadora del Conicet
8 Bioquímica, Laboratorio de Inmunología Molecular y Celular, Centro de Estudios Farmacológicos, Investigadora del Conicet

Acceso a la fuente original
International Immunopharmacology
http://www.sciencedirect.com/science/journal/15675769

ua40317