Marta Martins *

Autora invitada por SIIC

Este estudo reporta um método que utiliza placas de agar com brometo de etídeo e que permite a identificação de actividade de efluxo em estirpes bacterianas multirresistentes.

O MÉTODO DE AGAR COM BROMETO DE ETÍDEO PARA IDENTIFICAÇÃO DE MULTIRESISTÊNCIA MEDIADA POR EFLUXO EM ISOLADOS BACTERIANOS

O método apresentado é simples, de fácil execução e baixo custo, podendo portanto ser facilmente implementado ao nível laboratorial. Adicionalmente, permite a identificação de isolados bacterianos multi-resistentes que apresentam uma sobre-expressão dos sistemas de efluxo. Com base nesta metodologia é possível efectuar uma selecção rápida de um elevado número de isolados.

*Marta Martins
describe para SIIC los aspectos relevantes de su trabajo
IDENTIFICATION OF EFFLUX PUMP-MEDIATED MULTIDRUG-RESISTANT BACTERIA BY THE ETHIDIUM BROMIDE-AGAR CARTWHEEL METHOD
Institución principal de la investigación
*Universidade Nova de Lisboa (IHMT/UNL), Lisboa, Portugal
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Otros artículos escogidos

Referencias bibliográficas
(1) Gottlieb T, Nimmo GR. Antibiotic resistance is an emerging threat to public health: an urgent call to action at the Antimicrobial Resistance Summit 2011. Med J Aust. 2011 21;194(6):281-3. (2) Kumar A and Schweizer HP: Bacterial resistance to antibiotics: active efflux and reduced uptake. Adv Drug Deliv Rev 57: 1486–1513, 2005.(3) Piddock LJ: Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria. Clin Microbiol Rev 19: 382–402, 2006.(4) Nelson ML: Modulation of antibiotic efflux in bacteria. Curr Med Chem 1: 35–54, 2002. (5) Webber MA and Piddock LJV: The importance of efflux pumps in bacterial antibiotic resistance. J Antimicrob Chemother 51: 9–11, 2003.
(6) Zgurskaya HI and Nikaido H: Multidrug resistance mechanisms: drug efflux across two membranes. Mol Microb 37: 219–225, 2000. (7) Mortimer PG and Piddock LJ: A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus. J Antimicrob Chemother 28: 639–653, 1991. (8) Martins M, Santos B, Martins A, Viveiros M, Couto I, Cruz A, Pagès JM, Molnar J, Fanning S, Amaral L and Management Committee Members of Cost B16, European Commission/European Science Foundation: An instrument-free method for the demonstration of efflux pump activity of bacteria. In Vivo 20: 657–664, 2006. (9) Viveiros M, Dupont M, Rodrigues L, Couto I, Davin-Regli A, Martins M, Pagès JM and Amaral L: Antibiotic stress, genetic response and altered permeability of E. coli. PLoS ONE 2: e365, 2007. (10) Viveiros M, Martins M, Couto I, Rodrigues L, Spengler G, Martins A, Kristiansen JE, Molnar J and Amaral L: New methods for the identification of efflux mediated MDR bacteria, genetic assessment of regulators and efflux pump constituents, characterization of efflux systems and screening for inhibitors of efflux pumps. Curr Drug Targets 9: 760–778, 2008. (11) Martins M, Viveiros M, Couto I, Costa SS, Pacheco T, Fanning S, Pagès JM, Amaral L. Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method. In Vivo. 2011;25(2):171-8.

Otros artículos de Marta Martins

1. Martins, M. 2011. Targeting the Human Macrophage with Combinations of Drugs and Inhibitors of Ca(2+) and K(+) Transport to Enhance the Killing of Intracellular Multi-Drug Resistant M. tuberculosis (MDR-TB) - a Novel, Patentable Approach to Limit the Emergence of XDR-TB. Recent Pat. Anti-infect. Drug Discov. 6(2):110-117.

2.Martins, M., M. Viveiros, I. Couto, S.S. Costa, T. Pacheco, S. Fanning, J.M. Pagès, L. Amaral. 2011. Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method. In Vivo 25:171-178.

3.Martins, M., M. McCusker, L. Amaral, S. Fanning. 2011. Mechanisms of Antibiotic Resistance in Salmonella: Efflux Pumps, Genetics, Quorum Sensing and Biofilm Formation. Letters in Drug Design & Discovery 8: 114-123.

4.Amaral, L., M. Martins and M. Viveiros. 2011. Thioridazine: Alternative and Potentially Effective Therapy of the XDR-TB Patient. Letters in Drug Design & Discovery 8: 130-132.

5.Karczmarczyk, M., M. Martins, M . McCusker, S. Mattar, L. Amaral, N. Leonard, F. M. Aarestrup and S. Fanning. 2010. Characterization of antimicrobial resistance in Salmonella enterica food and animal isolates from Colombia: identification of a qnrB19-mediated quinolone resistance marker in two novel serovars. FEMS Microbiol Lett. 313:10-19.

6.Viveiros, M., M. Martins, I. Couto, L. Rodrigues, D. Machado, I. Portugal and L. Amaral. 2010. Molecular tools for rapid identification and novel effective therapy against MDRTB/XDRTB infections. Expert. Rev. Anti Infect. Ther. 8:465-80.

7. Martins, M., M. Viveiros, I. Couto, and L. Amaral. 2009. Targeting human macrophages for enhanced killing of intracellular XDR-TB and MDR-TB. Int. J. Tuberc. Lung Dis. 13:569-573.

8.Martins, M., M. Viveiros, J. Ramos, I. Couto, J. Molnar, M. Boeree, and L. Amaral. 2009. SILA 421, an inhibitor of efflux pumps of cancer cells, enhances the killing of intracellular extensively drug-resistant tuberculosis (XDR-TB). Int. J. Antimicrob. Agents 33:479-482.

9. Viveiros, M., M. Martins, I. Couto, L. Rodrigues, G. Spengler, A. Martins, J. E. Kristiansen, J. Molnar and L. Amaral. 2008. New Methods for the identification of efflux mediated MDR bacteria, genetic assessment of regulators and efflux pump constituents, characterization of efflux systems and screening for inhibitors of efflux pumps. Curr. Drug Targets. 9:760-778.

10. Martins, M., S. G. Dastidar, S. Fanning, J. E. Kristiansen, J. Molnar, J. M. Pagès, Z. Schelz, G. Spengler, M. Viveiros, and L. Amaral. 2008. Potential role of non-antibiotics (helper compounds) in the treatment of multidrug-resistant Gram-negative infections: mechanisms for their direct and indirect activities. Int. J. Antimicrob. Agents 31:198–208.

Para comunicarse con Marta Martins mencionar a SIIC como referencia:
marta.martins@ucd.ie

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Acerca del trabajo completo

O MÉTODO DE AGAR COM BROMETO DE ETÍDEO PARA IDENTIFICAÇÃO DE MULTIRESISTÊNCIA MEDIADA POR EFLUXO EM ISOLADOS BACTERIANOS

Título original en castellano
IDENTIFICAÇÃO DE MULTIRESISTÊNCIA MEDIADA POR EFLUXO EM BACTERIAS USANDO O METODO DE AGAR COM BROMETO DE ETIDEO

Autores
Marta Martins¹, Miguel Viveiros², Isabel Couto³, Sofia S. Costa⁴, Teresa Pacheco⁵, Séamus Fanning⁶, Jean-Marie Pagès⁷, Leonard Amaral⁸
1 Researcher, Unit Of Mycobacteriology, Upmm, Instituto de Higiene e Medicina Tropical and UCD Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Universidade Nova de Lisboa (IHMT/UNL), Lisboa, Portugal, Post-doc
2 Professor, Unit Of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (ihmt/unl), Lisboa, Portugal, Associate Professor
3 Professor, Unit Of Mycobacteriology, Instituto de Higiene e Medicina Tropical, (ihmt), And Centro de Recursos Microbiológicos (crem), Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa (unl), Portugal, Assistant Professor
4 Student, Unit Of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (ihmt/unl), Lisboa, Portugal, Phd Student
5 Microbiologista, Laboratório de Microbiologia, Hospital Egas Moniz, Lisboa, Portugal, Microbiologista No Laboratório de Microb
6 Professor Of Food Safety And Z, Ucd Centre For Food Safety, School Of Agriculture, Food Science And Veterinary Medicine,university College Dublin, Belfield, Dublin 4, Ireland, Professor
7 Professor, Département Ea2197 - Enveloppe Bactérienne, Perméabilité Et Antibiotiques, Université de la Méditerranée, Ufr/institut Faculté de Médecine, 13385 Marseille Cedex 05, France, Professor
8 Professor, Unit Of Mycobacteriology And Upmm, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (ihmt/unl), Lisbon, Portugal, Professor Emeritus

Acceso a la fuente original
In Vivo
http://www.iiar-anticancer.org/invivo/invivo_index.htm

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