Emmanuel Dias Nieto *

Autor invitado por SIIC

O DNA do parasita se encontra no líquor de pacientes infectados, e pode ser usado para um diagnóstico preciso da infecção

DIAGNÓSTICO MOLECULAR DA NEUROCISTICERCOSE

Os métodos atualmente disponíveis para o diagnóstico da neurocisticercose sofrem por alto custo (quando baseados em imagem) ou por reduzida especificidade ou sensibilidade (testes imunológicos). Pela primeira vez, apresentamos um teste molecular que permite o diagnóstico da neurocisticercose, com alta sensibilidade e alta especificidade.

*Emmanuel Dias Nieto
describe para SIIC los aspectos relevantes de su trabajo
TAENIA SOLIUM DNA IS PRESENT IN THE CEREBROSPINAL FLUID OF NEUROCYSTICERCOSIS PATIENTS AND CAN BE USED FOR DIAGNOSIS
Institución principal de la investigación
*Universidade de São Pablo, São Paulo, Brasil
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Referencias bibliográficas
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Del Brutto OH, Sotelo J. Neurocysticercosis: an update. Rev Infect Dis 1988; 10:1075-87.
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Machnicka B, Dziemian E, Zwiers C. Detection of Taenia saginata antigens in faeces by ELISA. Appl Parasitol 1996; 37:106-110.
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SES-SC: Secretaria de Estado da Saúde do Estado de Santa Catarina. Levantamento de dados sobre cisticercose em Santa Catarina de 1990 a 1995.

Otros artículos de Emmanuel Dias Nieto

Pena SDJ, Barreto G, Vago AR, Marco LA, Reinach FC, Dias Neto E, Simpson AJG. Sequence-specific "Gene signatures" obtained by PCR with single specific primers at low stringency (LSSP-PCR). Proccedings of the National Academies of Sciences USA 1994; 91:1946-1949.
Sanguinetti CJ, Dias Neto E, Simpson AJG. Recovery and eeamplification of PCR products from silver stained polyacrylamide gels. Biotechniques 1994; 17(4):3-6.
Dias Neto E, Harrop R, Corrêa Oliveira R, Wilson A, Pena SDJ, Simpson AJG. Minilibraries constructed from cDNA generated by arbitrarily primed RT-PCR: an alternative to normalized libraries for the efficient generation of ESTs from nanogram quantities of mRNA. Gene 1997; 186:135-142.
Dias Neto E, Corrêa RG, Verjovski Almeida S, Briones MRS, Nagai MA, Da Silva W Jr, Zago MA, Bordin S, Costa FF, Goldman GH, Carvalho AF, Matsukuma A, Baia GS, Simpson DH, Brunstein A, Oliveira PSL, Bucher P, Jongeneel CV, O’Hare M, Soares F, Brentani RR, Reis LFL, Souza SJ, Simpson AJG. Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Proccedings of the National Academies of Sciences USA 2000; 97:3491-3496.
Simpson AJG, Reinach FC, Arruda P, Abreu FA, Acencio M, Alvarenga R, Alves LMC, Araya JE, Baia GS, Baptista CS, Barros MH, Bonaccorsi ED, Bordin S, Bové JM, Briones MRS, Bueno MRP, Camargo AA, Camargo LEA, Carraro DM, Carrer H, Colauto NB, Colombo C, Costa FF, Costa MCR, Costa Neto CM, Coutinho LL, Cristofani M, Dias Neto E, Docena C, El-Dorry HFA, et al. The genome sequence of the plant pathogen Xylella fastidiosa. Nature 2000; 406:151-157.
Souza SJ, Camargo AA, Briones MRS, Costa FF, Nagai MA, Verjovski Almeida S, Zago MA, Andrade LEC, Carrer H, El-Dorry HFA, Valente, Brentani RR, Reis LFL, Dias Neto E, Simpson AJG. Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags. Proccedings of the National Academies of Sciences USA 2000; 97:12690-12693.
Pontes LA, Dias Neto E, Rabello A. PCR detection of Schistosoma mansoni DNA in human serum and feces. American Journal of Tropical Medicine and Hygiene 2002; 66:157-162.
Verjovski Almeida S, De Marco R, Martins EAL, Guimarães PEM, Ojopi EPB, Paquola ACM, Piazza JP, Nishiyama MY Jr, Kitajima JP, Adamson RE, Ashton P, Bonaldo MF, Coulson PS, Dillon GP, Faria LP, Gregório SP, Ho PL, Leite RA, Malaquias LCC, Marques RCP, Miyasato PA, Nascimento ALTO, Ohlweiler FP, Reis EM, Riberio MA, Sá RG, Stukart GC, Soares MB, Gargioni C, Kawano T, Rodrigues V, Madeira AMBN, Wilson RA, Menck CFM, Setúbal JC, Leite LCC, Dias Neto E. Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni. Nature Genetics 2003; 35:148-157.
Verjovski Almeida S, Leite LCC, Dias Neto E, Menck CFM, Wilson RA.. Insights into Schistosome metazoan and parasitic functions: perspectives for functional genomics. Trends in Parasitology 20:304-308.
Reis EM, Ojopi EPB, Alberto FL, Rahal P, Tsukumo F, Mancini U, Guimarães GS, Thompson GMA, Camacho C, Miracca E, Carvalho AL, Machado AA, Paquola ACM, Cerutti JM, Da Silva AM, Pereira GG, Valentini SR, Nagai MA, Kowalski LP, Verjovski Almeida S, Tajara EH, Dias Neto E, & Head and Neck annotation consortium. Large-scale transcriptome analyses reveal new genetic marker candidates of head, neck and thyroid cancer. Cancer Research 2005; 65:1693-1699.

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DIAGNÓSTICO MOLECULAR DA NEUROCISTICERCOSE

Título original en castellano
O DNA DE TAENIA SOLIUM ESTA PRESENTE NO LIQUIDO CEREBROESPINHAL DE PACIENTES COM NEUROCISTICERSOSE, E PODE SER USADO PARA DIAGNOSTICO

Autor
Emmanuel Dias Nieto¹
1 PhD, Laboratório de Neurociências, Instituto de Psiquiatria, Faculdade de Medicina, Universidade de São Pablo, São Paulo, Brasil, PhD

Acceso a la fuente original
European Archives of Psychiatry and Clinical Neuroscience
http://www.link.springer-ny.com/link/service/journals/00406/

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