Maria de Nazaré Correia Soeiro *

Autor invitado por SIIC

Através do uso de um modelo de cultivo primário de cardiomiócitos, o presente estudo contribuiu para um melhor entendimento sobre as alterações na via endocítica de células cardíacas frente a sua interação com Trypanosoma cruzi

TRYPANOSOMA CRUZI MODULA A VIA ENDOCÍTICA DE CARDIOMIÓCITOS IN VITRO

Células cardíacas infectadas pelo Trypanosoma cruzi apresentam perda de atividade endocítica possivelmente associada á regulação negativa de GTPases, tais como Rab7 e Rab11 (compartimentos endocíticos tardios e de reciclagem, respectivamente), e de moléculas efetoras como a EEA1, relacionada à Rab5a (envolvida no transporte entre compartimentos endocíticos iniciais).

*Maria de Nazaré Correia Soeiro
describe para SIIC los aspectos relevantes de su trabajo
TRYPANOSOMA CRUZI MODULATES THE EXPRESSION OF RABS AND ALTERS THE ENDOCYTOSIS IN MOUSE CARDIOMYOCYTES IN VITRO
Institución principal de la investigación
*Fundação Oswaldo Cruz, Rio de Janeiro, Brasil
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
1. Mousavi SA, Malerod L, Berg T, Kjeken R. Clathrin-dependent endocytosis. Biochem J 2004; 377(Pt 1):1-16.
2. Rabinovitch M. Professional and non-professional phagocytes: an introduction. Trends Cell Biol 1995; 5(3):85-87.
3. Soeiro MNC, Mota RA, Batista DGJ, Meirelles MNL. Endocytic pathway in mouse cardiac cells. Cell Struct Funct 2002; 27(6):469-478.
4. Hespanhol RC, Soeiro MNC, Meuser MB, Meirelles MNL, Corte-Real S. The expression of mannose receptors in skin fibroblast and their involvement in Leishmania (L.) amazonensis invasion. J Histochem Cytochem 2005; 53(1):35-44.
5. Marsh M, McMahon HT. The structural era of endocytosis. Science. 1999; 285(5425):215-220.
6. Conner SD, Schmid SL. Regulated portals of entry into the cell. Nature 2003; 422(6927):37-44.
7. Maxfield FR, McGraw TE. Endocytic recycling. Nat Rev Mol Cell Biol 2004; 5(2):121-132.
8. Johannes L, Lamaze C. Clathrin-dependent or not: is it still the question? Traffic 2002; 3(7):443-451.
9. Rabinowitz S, Horstmann H, Gordon S, Griffiths G. Immunocytochemical characterization of the endocytic and phagolysosomal compartments in peritoneal macrophages. J Cell Biol 1992; 116(1):95-112.
10. Desjardins M, Huber LA, Parton RG, Griffiths G. Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus. J Cell Biol 1994; 124(5):677-688.
11. Clague MJ. Molecular aspects of the endocytic pathway. Biochem J. 1998; 336 (Pt 2):271-282.
12. Van Vliet C, Thomas EC, Merino-Trigo A, Teasdale RD, Gleeson PA. Intracellular sorting and transport of proteins. Prog Biophys Mol Biol 2003; 83(1):1-45.
13. Miaczynska M, Zerial M. Mosaic organization of the endocytic pathway. Exp Cell Res 2002; 272(1):8-14.
14. Gruenberg J. The endocytic pathway: a mosaic of domains. Nat Rev Mol Cell Biol 2001; 2(10):721-730.
15. Zerial M, McBride H. Rab proteins as membrane organizersNat Rev Mol Cell Biol 2001; 2(2):107-117.
16. Haas A. Reprogramming the phagocytic pathway--intracellular pathogens and their vacuoles. Mol Membr Biol 1998; 15(3):103-121.
17. Hashim S, Mukherjee K, Raje M, Basu SK, Mukhopadhyay A. Live Salmonella modulate expression of Rab proteins to persist in a specialized compartment and escape transport to lysosomes. J Biol Chem 2000; 275(21):16281-16288.
18. Luhrmann A, Streker K, Schuttfort A, Daniels JJ, Haas A. Afipia felis induces uptake by macrophages directly into a nonendocytic compartment. Proc Natl Acad Sci USA 2001; 98(13):7271-7276.
19. Stein MP, Dong J, Wandinger-Ness A. Rab proteins and endocytic trafficking: potential targets for therapeutic intervention. Adv Drug Deliv Rev 2003; 55(11):1421-1437.
20. Walburger A, Koul A, Ferrari G, Nguyen L, Prescianotto-Baschong C, Huygen K, Klebl B, Thompson C, Bacher G, Pieters J. Protein kinase G from pathogenic mycobacteria promotes survival within macrophages. Science 2004; 304(5678):1800-1804.
21. Soeiro MNC, Costa e Silva Filho F, Meirelles MNL. Alterations in the surface charge of heart muscle cells during interaction with Trypanosoma cruzi. Cell Biophys 1995; 26(1):21-44.
22. Soeiro MNC, Paiva MM, Barbosa HS, Meirelles MNL, Araújo-Jorge TC. A cardiomyocyte mannose receptor system is involved in Trypanosoma cruzi invasion and is down-modulated after infection. Cell Struct Funct 1999; 24(3):139-149.
23. Bucci C, Thomsen P, Nicoziani P, McCarthy J, Van Deurs B. Rab7: a key to lysosome biogenesis. Mol Biol Cell 2000; 11(2):467-480.
24. Maxfield FR, McGraw TE. Endocytic recycling. Nat Rev Mol Cell Biol 2004; 5(2):121-132.
25. Soeiro MN, Mota RA, Batista DG, Pereira MC, Meirelles MN. Trypanosoma cruzi infection impairs the endocytosis of zymosan A by cardiomyocytes. Pathobiology 2002; 70(2):69-75.
26. Balligand JL, Ungureanu-Longrois D, Simmons WW, Pimental D, Malinskin TA, Kapturczakn M, Tahan Z, Lowensteinll CJ, Davidoff AJ, Kelly RA, Smith TW, Michel T. Cytokine-inducible nitric oxide synthase (iNOS) expression in cardiac myocytes. Characterization and regulation of iNOS expression and detection of iNOS activity in single cardiac myocytes in vitro. J Biol Chem 1994; 269(44):27580-27588.
27. Machado FS, Martins GA, Aliberti JC, Mestriner FL, Cunha FQ, Silva JS. Trypanosoma cruzi-infected cardiomyocytes produce chemokines and cytokines that trigger potent nitric oxide-dependent trypanocidal activity. Circulation 2000; 102(24):3003-3008.
28. Fichera LE, Albareda MC, Laucella SA, Postan M. Intracellular growth of Trypanosoma cruzi in cardiac myocytes is inhibited by cytokine-induced nitric oxide. release. Infect Immun 2004; 72(1):359-363.
29. Finkel MS, Oddis CV, Jacob TD, Watkins SC, Hattler BG, Simmons RL. Negative inotropic effects of cytokines on the heart mediated by nitric oxide. Science 1992; 257(5068):387-389.
30. Cabeza Meckert PM, Chambo JG, Laguens RP. Presence of cells producing antiheart autoantibodies in the inflammatory infiltrate of chronic chagasic myocarditis. Clin Immunol Immunopathol 1991; 60(1):137-144.
31. Alvarez Domínguez C, Stahl PD. Interferon-gamma selectively induces Rab5a synthesis and processing in mononuclear cells. J Biol Chem 1998; 273(51):33901-33904.
32. Hackstadt T. Redirection of host vesicle trafficking pathways by intracellular parasites. Traffic 2000; 1(2):93-99.
33. Seabra MC, Mules EH, Hume AN. Rab GTPases, intracellular traffic and disease. Trends Mol Med 2002; 8(1):23-30.
34. Chua J, Vergne I, Master S, Deretic V. A tale of two lipids: Mycobacterium tuberculosis phagosome maturation arrest. Curr Opin Microbiol 2004; 7(1):71-77.
35. Sibley LD, Andrews NW. Cell invasion by un-palatable parasites. Traffic 2000; 1(2):100-106.
36. Desjardins M, Descoteaux A. Inhibition of phagolysosomal biogenesis by the Leishmania lipophosphoglycan. J Exp Med 1997; 185(12):2061-2068.
37. Borges VM, Vannier-Santos MA, de Souza W. Subverted transferrin trafficking in Leishmania-infected macrophages. Parasitol Res 1998; 84(10):811-822.
38. Alvarez Domínguez C, Roberts R, Stahl PD. Internalized Listeria monocytogenes modulates intracellular trafficking and delays maturation of the phagosome. J Cell Sci 1997; 110:731-743.

Otros artículos de Maria de Nazaré Correia Soeiro

1. Souza EM, Menna-Barreto R, Araújojorge TC, Kumar A, Hu Q, Boykin DW, Soeiro M. Antiparasitic activity of aromatic diamidines is related to apoptosis-like death in Trypanosoma cruzi. Parasitology (London), 2006; 133:75-79.
2. Souza EM, Boykin DW, Oliveira GM, Kumar A, Hu Q, Soeiro M. Trypanocidal activity of the phenyl-substituted analogue of furamidine DB569 against Trypanosoma cruzi infection in vivo. JAC. In press, 2006.
3. Hespanhol RC, Soeiro M, Meirelles MNL, Batista MM, Côrtereal S. The expression of mannose-receptors in the skin fibroblast and their involvement in Leishmania (L.) amazonensis invasion. Journal of Histoichemistry and Cytochemistry 2005; 53(1):35-44.
4. Soeiro M, Souza EM, Stephens CE, Boykin DW. Aromatic diamidines as antiparasitic agents. Expert Opinion on Investigational Drugs 2005; 14(8):957-972.
5. Waghabi M, Keramidas M, Bailly S, Degrave W, Mendonça-Lima L, Soeiro M, Meirelles MNL, Paciornik S, Araújojorge TC, Feige JJ. Uptake of host cell transforming growth factor-b by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion. The American Journal of Pathology 2005; 167(4):993-1003.
6. Souza EM, Pons AH, Bailly C, Lansiaux A, Araújojorge TC, Soeiro M. Enzymatic markers for detection of cellular injury in primary cultures of mice cardiomyocytes. Memórias do Instituto Oswaldo Cruz 2004; 99(7):697-701.
7. Souza EM, Lansiaux A, Bailly C, Wilson W, Hu Q, Boykin DW, Batista MM, Araújojorge TC, Soeiro M. Phenyl substitution of furamidine markedly potentiates its antiparasitic activity against Trypanosoma cruzi and Leishmania (L) amazonensis. Biochemical Pharmacology 2004; 68(4):593-600.
8. Souza EM, Araújojorge TC, Bailly C, Lansiaux A, Batista MM, Oliveira GM, Soeiro M. Host and parasite apoptosis following Trypanosoma cruzi infection in in vitro and in vivo models. Cell Tissue & Research 2003; 314(2):223-235.
9. Waghabi M, Coutinho CML, Soeiro M, Feige JJ, Minoprio P, Leuven FV, Araújojorge TC. Increased Trypanosoma cruzi invasion and heart fibrosis development is associated with high TGF -levels in mice deficient in alpha-2-macroglobulin. Infection and Immunity 2002; 70:5115-5123.
10. Soeiro M, Mota RA, Batista DGJ, Pereira MCS, Meirelles MNS. Trypanosoma cruzi infection impairs zymosam uptake by cardiomyocytes. Pathobiology 2002; 70:69-75.

Para comunicarse con Maria de Nazaré Correia Soeiro mencionar a SIIC como referencia:
soeiro@ioc.fiocruz.br

---

Acerca del trabajo completo

TRYPANOSOMA CRUZI MODULA A VIA ENDOCÍTICA DE CARDIOMIÓCITOS IN VITRO



Autores
CF Silva¹, RA Mota², Maria de Nazaré Correia Soeiro³, Denise da Gama Jaen Batista⁴
1, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil
2, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil
3, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil, Pesquisador
4, FIOCRUZ, Rio de Janeiro, Brasil, Estudante

Acceso a la fuente original
Journal of Histochemistry & Cytochemistry
http://www.jhc.org/

ua40317