Maria Isabel Nogueira Cano *

Autora invitada por SIIC

PROTEINAS TELOMERICAS EM PROTOZOARIOS DO GENERO LEISHMANIA

Neste trabalho mostramos a identificaçao e caracterizaçao de tres proteinas telomericas de Leishmania amazonensis alem da presença de atividade de telomerase em extratos do parasita. Para isso utilizou-se ensaios cromatográficos, ensaios de interação proteína-DNA, testes de extensao de sequencias telomericas e espectrometria de massa.

*Maria Isabel Nogueira Cano
describe para SIIC los aspectos relevantes de su trabajo
IDENTIFICATION OF THREE PROTEINS THAT ASSOCIATE IN VITRO WITH THE LEISHMANIA (LEISHMANIA) AMAZONENSIS G-RICH TELOMERIC STRAND
Institución principal de la investigación
*Departamento de Patología Clínica Facultad de Ciencias Médicas Universidad Estatal de Campinas (UNICAMP) SP, Brasil, Campinas, Brasil
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Otros artículos de Maria Isabel Nogueira Cano

1. Fernández MF, Castellari RR, Conte FF, Gozzo F, Sabino AA, Pinheiro H, Novello JC, Eberlin MN, Cano MIN. (2004) Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand. European Journal of Biochemistry, 271, 3050-3063.

2. Cano MI, Blake JJ, Blackburn EH, Agabian N. (2002) A Trypanosoma brucei protein that binds G overhangs and co-purifies with telomerase activity. Journal of Biological Chemistry, 277, 896-906.

3. Cano MI. Telomere Biology of Kinetoplastida protozoa: The little we know (Opinion) (2001) Trends in Parasitology, 17, 9425-9429.

4. Chiurillo M, Cano MI, Franco da Silveira J, Ramirez JL. (1999) Structure and organization of telomeric and subtelomeric regions of chromosomes from the protozoan parasite Trypanosoma cruzi. Molecular and Biochemical Parasitology, 100, 173-183.

5. Cano MI, Dungan J, Agabian N, Blackburn EH. (1999) Telomerase in kinetoplastid parasitic protozoa. Proceedings of the National Academy of Sciences USA, 96, 3616-3621.

6. Cano MI, Cisalpino P, Galindo I, Ramirez JL, Mortara R, Franco da Silveira J. (1998) Separation of Paracoccidioides brasiliensis chromosome-sized DNA molecules by pulsed field gel electrophoresis. Journal of Clinical Microbiology, 36, 742-747.

7. Zingales B, Pereira ME, Oliveira RP, Almeida KA, Umezawa ES, Souto RP, Vargas N, Cano MI, Da Silveira JF, Nehme NS, Morel CM, Brener Z, Macedo A. (1997) Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener. Acta Tropica, 29, 1-15.

8. Araya J, Cano MI, Novak EM, Gomes HBM, Guevara P, Ramires JL, Franco da Silveira J. (1997) A novel reiterated family of interspersed DNA elements in the genome of Trypanosoma cruzi. Parasitology, 115, 563-570.

9. Cisalpino P, Puccia R, Yamauchi L, Cano MI, Franco da Silveira J, Travassos LR. (1995) Cloning, characterization and epitope expression of the major diagnostic antigen of Paracoccidioides brasiliensis. Journal of Biological Chemistry, 271, 4553-4560.

10. Cano MI, Gruber A, Vasquez M, Cortes A, Levin MJ, Gonzalez A, Degrave W, Rondinelli E, Zingales E, Ramirez Jl, Alonso C, Requema JM, Franco da Silveira J. (1995) Trypanosoma cruzi genome project: Molecular karyotype of clone CL Brener. Molecular and Biochemical Parasitology, 71, 273-278.


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Acerca del trabajo completo

PROTEINAS TELOMERICAS EM PROTOZOARIOS DO GENERO LEISHMANIA

Título original en castellano
Identificação de tres proteinas que se associam in vitro com a fita telomerica rica em G de Leishmania (Leishmania) amazonensis

Autor
Maria Isabel Nogueira Cano¹
1 Biomédica, Universidade Estadual de Campinas, Investigador

Acceso a la fuente original
European Journal of Biochemistry
http://www.ejb.org/

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