Paula Alves *

Autor invitado por SIIC

O trabalho desenvolvido demonstra a importância de reavaliar os protocolos convencionais para a criopreservação eficiente de sistemas celulares complexos, tais como culturas primárias de neuroesferas.

ENCAPSULAÇÃO EM ALGINATO COMO NOVA ESTRATÉGIA PARA A CRIOPRESERVAÇÃO DE NEUROESFERAS

Culturas primárias de neuroesferas de células do cérebro são modelos in vitro valiosos para neurotoxicologia e investigação fundamental. Neste trabalho foi desenvolvida uma estratégia integrada que permite a cultura por tempo prolongado e criopreservação de neuroesferas com elevada viabilidade e reduzido tempo de recuperação após o descongelamento.

*Paula Alves
describe para SIIC los aspectos relevantes de su trabajo
ALGINATE ENCAPSULATION AS A NOVEL STRATEGY FOR THE CRYOPRESERVATION OF NEUROSPHERES
Institución principal de la investigación
*Instituto de Biologia Experimental e Tecnológica, Únidade de Tecnologia de Células Animais / Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal, Oeiras, Portugal
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Referencias bibliográficas
Miranda JP, Rodrigues A, Tostões RM, Leite S, Zimmerman H, Carrondo MJ, Alves PM. Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors. Tissue Eng Part C Methods, 2010. http://dx.doi.org/10.1089/ten.tec.2009.0784.
Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM. Cryopreservation of adherent cells: strategies to improve cell viability and function after thawing. Tissue Engineering Part C, 15:1-14, 2009. http://de.doi.org/10.1089/ten.tec.2009.0784.
Sá Santos, S, Leite, SM, Sonnewald, U, Carrondo, MJT, Alves PM. Stirrer vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics, Journal of Neuroscience Research 85(15): 3386-3397, 2007. http://dx.doi.org10.1002/jnr.21409.
Malpique R, Katsen-Globa A, Carrondo MJT, Zimmermann H, Alves PM. Cryopreservation in micro-volumes: impact upon Caco-2 colon adenocarcinoma cells proliferation and differentiation. Biotechnology & Bioengineering 98:155-66, 2007. http://dx.doi.org/10.1002/bit.21382.
Serra M, Leite S, Brito C, Costa J, Carrondo MJT, Alves PM. A novel culture strategy for human stem cell proliferation and neuronal differentiation. Journal of Neuroscience Research 85(16):3557-3566, 2007. http://dx.doi.org/10.1002/jnr.21451.

Otros artículos de Paula Alves

Miranda JP, Rodrigues A, Tostões RM, Leite S, Zimmerman H, Carrondo MJ, Alves PM. Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors. Tissue Eng Part C Methods, 2010. http://dx.doi.org/10.1089/ten.tec.2009.0784.
Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM. Cryopreservation of adherent cells: strategies to improve cell viability and function after thawing. Tissue Engineering Part C 15:1-14, 2009. http://de.doi.org/10.1089/ten.tec.2009.0784
Sá Santos S, Leite SM, Sonnewald U, Carrondo MJT, Alves PM. Stirrer vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics. Journal of Neuroscience Research 85(15):3386-3397, 2007. http://dx.doi.org10.1002/jnr.21409.
Malpique R, Katsen-Globa A, Carrondo MJT, Zimmermann H, Alves PM. Cryopreservation in micro-volumes: impact upon Caco-2 Colon Adenocarcinoma cells proliferation and differentiation, Biotechnology & Bioengineering 98:155-66, 2007. http://dx.doi.org/10.1002/bit.21382.
Serra M, Leite S, Brito C, Costa J, Carrondo MJT, Alves PM. A novel culture strategy for human stem cell proliferation and neuronal differentiation. Journal of Neuroscience Research 85(16):3557-3566, 2007. http://dx.doi.org/10.1002/jnr.21451.
Amaral AI, Teixeira AP, Martens S, Sousa MFQ, Bernal V, Alves PM. Metabolic alterations induced by ischemia in primary cultures of astrocytes: merging (13)C NMR spectroscopy and metabolic flux analysis. Journal of Neurochemistry 113(3):48-735, 2010. http://dx.doi.org/10.1111/j.1471-4159.2010.06672.
Tostões RM, Leite SB, Miranda JP, Sousa MFQ, Wang DIC, Carrondo MJT, Alves PM. Perfusion of 3D encapsulated hepatocytes - a synergistic effect enhancing long term functionality in bioreactors. Biotechnology and Bioengineering, 2010.
Serra M, Brito C, Sousa MFQ, Jensen J, Strehl R, Hyllner J, Tostoes R, Clemente J, Carrondo MJT Alves PM. Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control. Journal of Biotechnology 148:208-215, 2010.
Queiroga C, Martel C, Brenner C, Alves PM, Vieira H. Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilisation and apoptosis. Journal Biological Chemistry 285:17077-17088, 2010.
Amaral AI, Teixeira A, Martens S, Bernal V, Sousa MFQ, Alves PM. Metabolic alterations induced by ischemia in primary cultures of astrocytes: merging 13C NMR spectroscopy and metabolic flux analysis. Journal of Neurochemistry 113:735-748, 2010.

Para comunicarse con Paula Alves mencionar a SIIC como referencia:
marques@ibet.pt

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Acerca del trabajo completo

ENCAPSULAÇÃO EM ALGINATO COMO NOVA ESTRATÉGIA PARA A CRIOPRESERVAÇÃO DE NEUROESFERAS

Título original en castellano
ENCAPSULAÇÃO EM ALGINATO COMO NOVA ESTRATEGIA PARA A CRIOPRESERVAÇÃO DE NEUROESFERAS

Autores
Paula Alves¹, Rita Malpique², Heiko Zimmermann³, Fredrika Ehrhart⁴, Catarina Brito⁵
1 Professora e Investigadora, Instituto de Biologia Experimental e Tecnológica, Únidade de Tecnologia de Células Animais / Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal, Oeiras, Portugal, Directora de Departamento
2 Investigadora, Ibet/itqb-unl, Portugal, Pos-doc
3 Professor e Investigador, Ibmt-fraunhofer, Germany, Director de Departamento
4 Investigadora, Ibmt-fraunhofer, Germany
5 Investigadora, Ibet, Portugal, Anabrito@itqb.unl.pt

Acceso a la fuente original
Tissue Engineering: Part C - Methods
http://www.liebertonline.com/loi/tec?cookieSet=1

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