Cristian Ruiz *

Autor invitado por SIIC

La acción de los ácidos grasos saturados sobre las lipasas microbianas ensayadas varía en función de la estructura tridimensional de las enzimas

EFECTO DE LOS ACIDOS GRASOS SATURADOS SOBRE DIVERSAS LIPASAS MICROBIANAS

Se describe un nuevo microensayo que permite determinar con gran eficacia la inhibición de la actividad lipasa. Los ácidos grasos saturados provocan la inhibición de la lipasa de Candida rugosa, mientras que las lipasas de especies relacionadas con Bacillus muestran una respuesta variable, en función de su estructura tridimensional

*Cristian Ruiz
describe para SIIC los aspectos relevantes de su trabajo
ACTIVATION AND INHIBITION OF CANDIDA RUGOSA AND BACILLUS-RELATED LIPASES BY SATURATED FATTY ACIDS, EVALUATED BY A NEW COLORIMETRIC MICROASSAY
Institución principal de la investigación
*Department of Pharmacology of Natural Substances and General Physiology, University of Rome ?La Sapienza?, Roma, Italia, Barcelona, España
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Referencias bibliográficas
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Reetz, Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases, Annu. Rev. Microbiol. 53 (1999) 315– 351.14 V. Khalameyzer, I. Fischer, U.T. Bornscheuer, J. Altenbuchner, Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones, Appl. Environ. Microbiol. 65 (1999) 477–482.15 C. Ruiz, F.I.J. Pastor, P. Diaz, Isolation and characterization of Bacillus sp. BP-6 LipA, a ubiquitous lipase among mesophilic Bacillus species, Lett. Appl. Microbiol. 37 (2003) 354– 359.16 T. Eggert, G. van Pouderoyen, B.W. Dijkstra, K.-E. Jaeger, Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional struc-ture, FEBS Lett. 502 (2001) 89– 92.17 T. Eggert, U. Brockmeier, M.J. Droge, W.J. Quax, K.-E. 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Otros artículos de Cristian Ruiz

N. Prim, A. Blanco, J. Martinez, F.I.J. Pastor, P. Diaz, EstA, a gene coding for a cell-bound esterase from Paenibacillus sp. BP-23, is a new member of the bacterial subclass of type B carboxylesterases, Res. Microbiol. 151 (2000) 303– 312.C. Ruiz, F.I.J. Pastor, P. Diaz, Isolation and characterization of Bacillus sp. BP-6 LipA, a ubiquitous lipase among mesophilic Bacillus species, Lett. Appl. Microbiol. 37 (2003) 354– 359.N. Prim, F.I.J. Pastor, P. Diaz, Cloning and characterization of a bacterial cell-bound type B carboxylesterase from Bacillus sp. BP-7, Curr. Microbiol. 42 (2001) 237– 240.C. Ruiz, A. Blanco, F.I.J. Pastor, P. Diaz, Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase, FEMS Microbiol. Lett. 217 (2002) 263– 267.E. Grippa, R. Valla, L. Battinelli, G. Mazzanti, L. Saso, B. Silvestrini, Inhibition of Candida rugosa lipase by berberine and structurally related alkaloids, evaluated by high-performance liquid chromatography, Biosci. Biotechnol. Biochem. 63 (1999) 1557– 1562.M. Sanchez, N. Prim, F. Randez-Gil, F.I.J. Pastor, P. Diaz, Engineering of baker’s yeasts, E. coli and Bacillus hosts for the production of Bacillus subtilis Lipase A, Biotechnol. Bioeng. 78 (2002) 339–345.Prim, N., Sánchez, M., Ruiz, C., Pastor, F.I.J., Diaz, P. (2003). Use of methylumbeliferyl-derivative substrates for lipase activity characterization. J. Mol. Cat. B-Enz. 22:339-346.Firuzi O, Giansanti L, Vento R, Seibert C, Petrucci R, Marrosu G, Agostino R, Saso L. (2003) Hypochlorite scavenging activity of hydroxycinnamic acids evaluated by a rapid microplate method based on the measurement of chloramines. J Pharm Pharmacol. 55(7):1021-7.Bonanni G, Saso L.(2003) Evaluation of the adherence to the asthma guidelines by the administration of a questionnaire in community pharmacies. Minerva Med. 2003 Feb;94(1):29-40.Ruiz C, Falcocchio S, Xoxi E, Javier Pastor FI, Diaz P, Saso L (2004). Activation and inhibition of Candida rugosa and Bacillus-related lipases by saturated fatty acids, evaluated by a new colorimetric microassay. Biochim Biophys Acta1672(3):184-191.

Para comunicarse con Cristian Ruiz mencionar a SIIC como referencia:
pdiaz@ub.edu

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Acerca del trabajo completo

EFECTO DE LOS ACIDOS GRASOS SATURADOS SOBRE DIVERSAS LIPASAS MICROBIANAS

Título original en castellano
ACTIVACION E INHIBICION POR ACIDOS GRASOS SATURADOS DE LAS LIPASAS DE CANDIDA RUGOSA Y DE ESPECIES RELACIONADAS CON BACILLUS, EVALUADAS MEDIANTE UN NUEVO MICROENSAYO COLORIMETRICO

Autores
Cristian Ruiz¹, Serena Falcocchio², Entela Xoxi³, F.I. Javier Pastor⁴, Pilar Díaz⁵, Luciano Saso⁶
1 Estudiante de Doctorado, Departamento de Microbiología. Universidad de Barcelona, Investigador Contratado
2 Investigadora, Department of Pharmacology of Natural Substances and General Physiology, Univers, Investigadora Contratada
3 Estudiante de Doctorado, Department of Pharmacology of Natural Substances and General Physiology, Becaria Investigación
4 Biólogo, Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Profesor Titular de Universidad
5 Bióloga, Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Profesora Titular de Universidad
6 Farmaceutico, Department df Pharmacology df Natural Substances and General Physiology, Univers, Académico/ Investigador

Acceso a la fuente original
BBA General Subjects
http://www.elsevier.com/wps/find/journaldescription.cws_home/506066/description

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