Bruno Gonzalez-Zorn *

Autor invitado por SIIC

El trabajo alerta sobre la adquisición cromosómica de una metiltransferasa de resistencia a aminoglucósidos en una bacteria patógena

COSTO BIOLÓGICO E INTERFERENCIA ENTRE METILASAS ADQUIRIDAS Y ENDÓGENAS

En este trabajo observamos que la adquisición cromosómica de una metiltransferasa del ARNr 16S de resistencia a aminoglucósidos no supone un costo biológico adicional para la bacteria, metilando todos los ribosomas incluso en ausencia de antibióticos. Además, esta metilación desplaza metilaciones endógenas adyacentes altamente conservadas a lo largo de la evolución.

*Bruno Gonzalez-Zorn
describe para SIIC los aspectos relevantes de su trabajo
FITNESS COST AND INTERFERENCE OF ARM/RMT AMINOGLYCOSIDE RESISTANCE WITH THE RSMF HOUSEKEEPING METHYLTRANSFERASES
Institución principal de la investigación
*Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, España
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
1. Andersen NM, Douthwaite S. YebU is a m5C methyltransferase specific for 16S rRNA nucleotide 1407. J Mol Biol 359:777-786, 2006.
2. Andersen TE, Porse BT, Kirpekar F. A novel partial modification at C2501 in Escherichia coli 23S ribosomal RNA. RNA 10:907-913, 2004.
3. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-8, 2006.
4. Basturea GN, Rudd KE, Deutscher MP. dentification and characterization of RsmE, the founding member of a new RNA base methyltransferase family. RNA 12:426-434, 2006.
5. Cherepanov PP, Wackernagel W. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158:9-14, 1995.
6. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 19th ed. Approved standard M100-S19. CLSI, Wayne, PA, 2009.
7. Cubrilo S, Babi F, Douthwaite S, Maravi J, Vlahovicek G. The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site. RNA 15:1492-1497, 2009.
8. Cundliffe E. How antibiotic-producing organisms avoid suicide. Annu Rev Microbiol 43:207-233, 1989.
9. Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci 97:6640-6645, 2000.
10. Davis MA, Baker KNK, Orfe LH, Shah DH, Besser TE, Call DR. Discovery of a gene conferring multiple aminoglycoside resistance in Escherichia coli. Antimicrob. Agents Chemother 54:2666-2669, 2010.
11. Doi Y, Yokoyama K, Yamane K, Wachino J, Shibata N, Yagi T, Shibayama K, Kato H, Arakawa Y. Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosides. Antimicrob Agents Chemother 48:491-496, 2004.
12. Doi Y, De Oliveira Garcia D, Adams J, Paterson DL. Coproduction of novel 16S rRNA methylase RmtD and metallo beta-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from Brazil. Antimicrob Agents Chemother 51:852-856, 2007.
13. Folster JP, Rickert R, Barzilay EJ, Whichard JM. Identification of the aminoglycoside resistance determinants armA and rmtC among non-Typhi Salmonella isolates from humans in the United States. Antimicrob Agents Chemother 53:4563-4564, 2009.
14. François B, Russell RJM, Murray JB, Aboull-ela F, Masquida B, Vicens Q, Westhof E. Crystal structures of complexes between aminoglycosides and decoding A site oligonucleotides: Role of the number of rings and positive charges in the specific binding leading to miscoding. Nucleic Acids Res 33:5677-5690, 2005.
15. Galimand M, Courvalin P, Lambert T. Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob Agents Chemother 47:2565-71, 2003.
16. González Zorn B, Teshager T, Casas M, Porrero MC, Moreno MA, Courvalin P, Domínguez L. ArmA and aminoglycoside resistance in Escherichia coli. Emerg Infect Dis 11:954-956, 2005.
17. Granier SA, Hidalgo L, San Millan A, Escudero JA, Gutierrez B, Brisabois A, González Zorn B. ArmA methyltransferase in a monophasic Salmonella enterica isolate from food. Antimicrob. Agents Chemother 55:5262-6, 2011.
18. Han Q, Zhao Q, Fish S, Simonsen KB, Vourloumis D, Froelich JM, Wall D, Hermann T. Molecular recognition by glycoside pseudo base pairs and triples in an apramycin-RNA complex. Angew Chem Int Ed Engl 44:2694-700, 2005.
19. Helser TL, Davies JE, Dahlberg JE. Mechanism of kasugamycin resistance in Escherichia coli. Nat New Biol 235:6-9, 1972.
20. Hopkins KL, Escudero JA, Hidalgo L, González Zorn B. 16S rRNA methyltransferase RmtC in Salmonella enterica serovar Virchow. Emerg Infect Dis 16:712-715, 2010.
21. Kimura S, Suzuki T. Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA. Nucleic Acids Res 38:1341-1352, 2010.
22. Kirpekar F, Douthwaite S, Roepstorff P. Mapping posttranscriptional modifications in 5S ribosomal RNA by MALDI mass spectrometry. RNA 6:296-306, 2000.
23. Kuhlman TE, Cox EC. Site-specific chromosomal integration of large synthetic constructs. Nucleic Acids Res 38:e92, 2010.
24. Liou GF, Yoshizawa S, Courvalin P, Galimand M. Aminoglycoside resistance by ArmA-mediated ribosomal 16S methylation in human bacterial pathogens. J Mol Biol 359:358-364, 2006.
25. Ofengand J, Del Campo M. Modified nucleosides in Escherichia coli ribosomal RNA. In EcoSal-Escherichia coli and Salmonella: Cellular and molecular biology (ed. R Curtiss), Chap. 4.6.1. ASM. Press, Washington, DC, 2004.
26. Ogle JM, Ramakrishnan V. Structural insights into translational fidelity. Annu Rev Biochem 74:129-177, 2005.
27. San Millan A, García Cobos S, Escudero JA, Hidalgo L, Gutiérrez B, Carrilero L, Campos J, González Zorn B. Haemophilus influenzae clinical isolates with plasmid pB1000 bearing blaROB-1: fitness cost and interspecies dissemination. Antimicrob Agents Chemother 54:1506-1511, 2010.
28. Schmitt E, Galimand M, Panvert M, Courvalin P, Mechulam Y. Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases. J Mol Biol 388:570-852, 2009.
29. Shandrick S, Zhao Q, Han Q, Ayida BK, Takahashi M, Winters GC, Simonsen KB, Vourloumis D, Hermann T. Monitoring molecular recognition of the ribosomal decoding site. Angew Chem Int Ed Engl 43:3177-3182, 2000.
30. Shaw KJ, Rather PN, Hare RS, Miller GH. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside- modifying enzymes. Microbiol Rev 57:138-163, 1993.
31. Snyder L, Champness W. Plasmids, molecular genetics of bacteria. ASM Press, Washington, DC, pp. 208-209, 2007.
32. Vincens Q, Westhof E. RNA as a drug target: the case of aminoglycosides. Chembiochem 4:1018-1023, 2003.
33. Wachino J, Yamane K, Kimura K, Shibata N, Suzuki S, Ike Y, Arakawa Y. Mode of transposition and expression of 16S rRNA methyltransferase gene rmtC accompanied by ISEcp1. Antimicrob Agents Chemother 50:3212-3215, 2006.
34. World Health Organisation. Critically important 1 antimicrobials for human medicine: categorization for the development of risk management strategies to contain antimicrobial resistance due to non-human antimicrobial use: report of the second WHO expert meeting, 29-31 May 2007, Copenhagen, Denmark. World Health Organisation, Geneva, Switzerland. Available from http://www.who.int/foodborne_disease/resistance/antimicrobials_human.pdf, 2007.
35. Yokoyama K, Doi Y, Yamane K, Kurokawa H, Shibata N, Shibayama K, Yagi T, Kato H, Arakawa Y. Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa. Lancet 362:1888-1893, 2003.
36. Zhang W,Dunkle JA, Cate JH. Structures of the ribosome in intermediate states of ratcheting. Science 325:1014-1017, 2009.

Otros artículos de Bruno Gonzalez-Zorn

Gutiérrez B, Douthwaite S, González Zorn B. Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs. RNA Biology 10(8):1324-32, 2013.
Gutiérrez B, Herrera León S, Escudero JA, Hidalgo L, González Sanz R, Arroyo M, San Millán A, Echeita MA, González Zorn B. Novel genetic environment of qnrB2 associated with TEM-1 and SHV-12 on pB1004, an IncHI2 plasmid, in Salmonella Bredeney BB1047 from Spain. J Antimicrob Chemother 64:1334-6, 2009.
San Millán A, Escudero JA, Gutiérrez B, Hidalgo L, García N, Llagostera M, Domínguez L, González Zorn, B. Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids. Antimicrob Agents Chemother 53:3399-404, 2009.
Hidalgo L, Hopkins KL, Gutiérrez B, Ovejero CM, Shukla S, Douthwaite S, Prasad KN, Woodford N, González Zorn B. Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK. J Antimicrob Chemother 68:1543-50, 2013.
Hidalgo L, Gutiérrez B, Ovejero CM, Carrilero L, Matrat S, Saba CKS, Santos López A, Thomas López D, Hoefer A, Santurde G, Martín Espada C, González Zorn B. Klebsiella pneumoniae ST11 from companion animals bearing ArmA methyltransferase, DHA-1 beta-lactamase and QnrB4. Antimicrob Agents Chemother 57:4532-34, 2013.

Para comunicarse con Bruno Gonzalez-Zorn mencionar a SIIC como referencia:
bgzorn@ucm.es

---

Acerca del trabajo completo

COSTO BIOLÓGICO E INTERFERENCIA ENTRE METILASAS ADQUIRIDAS Y ENDÓGENAS

Título original en castellano
COSTE BIOLOGICO E INTERFERENCIA DE LAS METILASAS DE RESISTENCIA DE LA FAMILA ARM/RMT CON LA METILASA ENDOGENA RSMF

Autores
Bruno Gonzalez-Zorn¹, Belen Gutierrez²
1 Profesor de Universidad, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, España, Director de Departamento
2 Veterinario, Facultad de Veterinaria, Universidad Complutense de Madrid, Investigadora Postdoctoral

Acceso a la fuente original
Antimicrobial Agents and Chemotherapy
http://aac.asm.org/

ua40317