María Belén Prados *

Autora invitada por SIIC

MECANISMOS REGULATORIOS DE LA N-GLICOSILACIÓN DE LA IGG MEDIADOS POR PROGESTERONA

La progesterona regula la relación STT3-A/STT3-B, incrementando la N-glicosilación de la IgG1 secretada por el hibridoma 112D5. Este trabajo constituye la primera evidencia de que las isoformas de STT3 pueden ser moduladas hormonalmente y apoya la idea que la completa N-glicosilación de las proteínas requiere la cooperación entre ambas isoformas de la subunidad catalítica de OST.

*María Belén Prados
describe para SIIC los aspectos relevantes de su trabajo
PROGESTERONE INDUCES A SWITCH IN OLIGOSACCHARYLTRANSFERASE ISOFORM EXPRESSION: CONSEQUENCES ON IGG N-GLYCOSYLATION
Institución principal de la investigación
*Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini" (ININCA), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina
Imprimir nota

Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
1. Coloma MJ, Clift A, Wims L, Morrison S J. The role of carbohydrate in the assembly and function of polymeric IgG. Mol Immunol 2000; 37(18):1081-1090.
2. Jefferis R, Lund J, and Pound JD. IgG-Fc-mediated effector functions: molecular definition of interaction sites for effector ligands and the role of glycosylation. Immunol Rev, 1998; 163:59-76.
3. Mullinax F, Mullinax G. Abnormality of IgG structure in rheumatoid arthritis and systemic lupus erythematosus. Arthritis Rheum 1975; 18:417-418.
4. Tao and Morrison. Studies of aglycosylated mouse-human IgG. Role of carbohydrate in the structure and effector functions mediated by the human IgG constant region. J Immunol 1989; 143:2595-2601.
5. Tomana M, Schrohenloher R, Koopman W, Alarcon G, Paul W. Abnormal glycosylation of serum IgG from patients with chronic inflammatory diseases. Arthritis Reum 1988; 31:333-338.
6. Wright A, Tao MH, Kabat EA and Morrisson SL. Antibody variable region glycosylation: position effects on antigen binding and carbohydrate structure. EMBO J 1991; 10:2717-2723.
7. Co MS, Scheimberg N, Avdalovic NM, Mc Graw K, Vásquez M, Caron P and Queen C. Genetically engineered deglycosylation of the variable domain increases the affinity of an anti-CD33 monoclonal antibody. Mol Immuno. 1993; 30:1361-1371.
8. Margni R and Binaghi R. Non-precipitating asymmetric antibodies. Annu Rev Immunol 1988; 6:535-554.
9. Morelli L, Plotkin L, Leoni J, Fossati C and Margni R. Analysis of oligosaccharides involved in the asymmetrical glycosylation of IgG monoclonal antibodies. Mol Immunol 1993; 30:695-700.
10. Margni R. Are the non-precipitating asymmetric antibodies autoprotective and regulatory antibodies? Res Immunol (Inst. Pasteur) 1989; 140:725-729.
11. Margni R and Malan Borel I. Paradoxical behavior of asymmmetric IgG antibodies. Immunol Reviews 1998; 163:77-87.
12. Jacquemin M, Radcliffe CM, Lavend’homme R, Wormald MR, Vanderelst L, Wallays G, Dewaele J, Collen D, Vermylen J, Dwek RA, Saint-Remy JM, Rudd PM and Dewerchin M. Variable region heavy chain glycosylation determines the anticoagulant activity of a factor VIII antibody. Journal of Thrombosis and Haemostasis 2006; 4:1047–1055
13. Omtvedt LA, Bailey D, Renouf DV, Davies MJ, Paramonov NA, Haavik S, Husby G, Sletten K, Hounsell EF. Glycosylation of immunoglobulin light chains associated with amyloidosis. Amyloid 2000; (7) 4:227-244.
14. Canellada A, Blois S, Gentile T, Margni R. In vitro modulation of protective antibody responses by estrogen, progesterone and interleukin-6. Am J Reprod Immunol 2002; 47:8735-8920.
15. Gutierrez G, Malan Borel I, Margni RA. The regulatory placental factor involved in asymmetric IgG antibody synthesis responds to IL-6 features. J Reprod Immunol 2001; 49:21-32.
16. Miranda S, Canellada A, Gentile T and Margni R. Interleukin-6 and dexamethasone modulate in vitro asymmetric antibodies synthesis and UDP-Glc glycoprotein glucosyltransferase activity. J Reprod Immunol 2005; 66:141-150.
17. Szereday L, Varga P, and Szekeres-Bartho J. Cytokine production in pregnancy. Am J Reprod Immunol 1997; 38:418–422.
18. Kelemen K, Bognar I, Paal M, Szekeres-Bartho J. A progesterone-induced protein increases the synthesis of asymmetric antibodies. Cell Immunol 1996; 167(1):129-34.
19. Lachmann M, Gelbmann D, Kalman E, Polgar B, Buschle M, Gabain A, Szekeres-Bartho J, and Nagy E. PIBF (progesterone induced blocking factor) is overexpressed in highly proliferating cells and associated with the centrosome. Int J Cancer 2004; 112:51–60.
20. Kornfeld R and Kornfeld S. Assembly of asparagine linked oligosaccharides. Ann Rev Biochem 1985; 54:631-64.
21. Kelleher D, Karaoglu D, Mandon E and Gilmore R. Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties. Mol Cell 2003; 19:101-111.
22. Ruiz-Canada C, Kelleher DJ, Gilmore R. Cotranslational and Posttranslational N-Glycosylation of Polypeptides by Distinct Mammalian OST Isoforms. Cell 2009; 136:272–283.