Begoña Ochoa Olascoaga *

Autora invitada por SIIC

El trabajo asigna un papel en el metabolismo hepático de los lípidos a SND p102, una proteína de función desconocida homóloga a un coactivador transcripcional

LA PROTEÍNA SND P102 PROMUEVE LA SECRECION DE FOSFOLIPIDOS EN LIPOPROTEINAS

La proteína de rata SND p102, conservada evolutivamente, se expresa en compartimentos no nucleares de los hepatocitos y parece regular la cantidad de fosfolípidos que éstos secretan en las lipoproteínas, sin afectar la secreción de triglicéridos, colesterol (libre o esterificado), apoB y apoA-I.

*Begoña Ochoa Olascoaga
describe para SIIC los aspectos relevantes de su trabajo
OVEREXPRESSION OF SND P102, A RAT HOMOLOGUE OF P100 COACTIVATOR, PROMOTES THE SECRETION OF LIPOPROTEIN PHOSPHOLIPIDS IN PRIMARY HEPATOCYTES
Institución principal de la investigación
*, Lejona, España, Lejona, España
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Comprar este artículo
Otros artículos escogidos

Referencias bibliográficas
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2 Yang J, Aittomäki S, Pesu M, Carter K, Saarinen J, Kalkkinen N, Kieff E, Silvennoinen O. Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II. EMBO J 21:4950-4958, 2002.
3. Paukku K, Yang J, Silvennoinen O. Tudor and nuclease-like domains containing protein p100 function as coactivators for signal transducer and activator of transcription 5. Mol Endocrinol 17:1805-1814, 2003.
4. Keenan TW, Winter S, Rackwitz HR, Heid HW. Nuclear coactivator protein p100 is present in endoplasmic reticulum and lipid droplets of milk secreting cells. Biochim Biophys Acta 1523:84-90, 2000.
5. Zhao CT, Shi KH, Su Y, Liang LY, Yan Y, Postlethwait J, Meng AM. Two variants of zebrafish p100 are expressed during embryogenesis and regulated by Nodal signaling. FEBS Lett 543:190-195, 2003.
6. Abe S, Sakai M, Yagi K, Hagino T, Ochi K, Shibata K, Davies E. A Tudor protein with multiple SNc domains from pea seedlings: cellular localization, partial characterization, sequence analysis, and phylogenetic relationships. J Exp Botany 54:971-983, 2003.
7. Callebaut I, Mornon JP. The human EBNA-2 coactivator p100: multidomain organization and relationship to the staphylococcal nuclease fold and to the Tudor protein involved in Drosophila melanogaster development. Biochem J 321:125-132, 1997.
8. Ponting CP. P100, a transcriptional coactivator, is a human homologue of staphylococcal nuclease. Protein Science 6:459-463, 1997.
9. Lienard P, Riviere M, Van Vooren P, Szpirer C, Szpirer J. Assignment of SND1, the gene encoding coactivator p100, to human chromosome 7q31.3 and rat chromosome 4q23 by in situ hybridization. Cytogenet Cell Genet 90:253-254, 2000.
10. Broadhurst MK, Lee RSF, Hawkins S, Wheeler TT. The p100 EBNA-2 coactivator: a highly conserved protein found in a range of exocrine and endocrine cells and tissues in cattle. Biochim Biophys Acta 1681:126-133, 2005.

Otros artículos de Begoña Ochoa Olascoaga

Isusi E, Aspichueta P, Liza M, Hernández ML, Díaz C, Hernández G, Martínez MJ, Ochoa B. Short- and long-term effects of atorvastatin, lovastatin and simvastatin on the cellular metabolism of cholesteryl esters and vldl secretion in rat hepatocytes. Atherosclerosis 153:283-294, 2000.
Fresnedo O, López de Heredia M, Martínez MJ, Cristóbal S, Rejas MT, Cuezva JM, Ochoa B. Immunolocalization of a novel cholesteryl ester hydrolase in the endoplasmic reticulum of murine and human hepatocytes. Hepatology 33:662- 667, 2001.
Botham KM, Maldonado EN, Chico Y, Zeng X, Avella M, Ochoa B. The influence of chylomicron remnants on cholesteryl ester metabolism in cultured rat hepatocytes: comparison of the effects of particles enriched in n-3 or n-6 polyunsaturated fatty acids. Biochim Biophys Acta 1534:96-109, 2001.
Mancheño JM, Pernas MA, Martínez MJ, Ochoa B, Rúa ML, Hermoso JA. Structural insights into the lipase/esterase behavior in the Candida rugosa lipases family: crystal structure of the lipase 2 isoenzyme at 1.97 a resolution. Journal of Molecular Biology 332:1059-1069, 2003.
Napolitano M, Bravo E, Avella M, Chico Y, Ochoa B, Botham KM, Rivabene R. The fatty acid composition of chylomicron remnants influences their propensity to oxidation. Nutr Metab Cardiov Dis 14:241-247, 2004.
Pérez S, Maldonado EN, Aspichueta P, Ochoa B, Chico Y. Differential modulation of prostaglandin receptor ARNm abundance by prostaglandins in primary cultured rat hepatocytes. Molecular and Cellular Biochemistry 266:183-189, 2004.
Aspichueta P, Pérez S, Ochoa B, Fresnedo O. Endotoxin promotes preferential periportal upregulation of very low density lipoprotein secretion in the rat liver. Journal Lipid Research 46:1017-1026, 2005.
Pérez S, Aspichueta P, Ochoa B, Chico Y. The 2-series prostaglandins suppress VLDL secretion in an inflammatory condition-dependent manner in primary rat hepatocytes. Biochim Biophys Acta 1761:160-171, 2006.
Aspichueta P, Pérez Agote B, Pérez S, Ochoa B, Fresnedo O. Impaired response of VLDL lipid and apob secretion to endotoxin in the fasted rat liver. J Endotoxin Res 12:181-191, 2006.
Palacios L, Ochoa B, Gómez Lechón MJ, Castell JV, Fresnedo O. Overexpression of SND p102, a rat homologue of p100 coactivator, promotes the secretion of lipoprotein phospholipids in primary hepatocytes. Biochim Biophys Acta 1761:698-708, 2006.


Para comunicarse con Begoña Ochoa Olascoaga mencionar a SIIC como referencia:
begona.ochoa@ehu.es

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Acerca del trabajo completo

LA PROTEÍNA SND P102 PROMUEVE LA SECRECION DE FOSFOLIPIDOS EN LIPOPROTEINAS

Título original en castellano
LA SOBREEXPRESION DE SND P102, UN HOMOLOGO DE RATA DEL COACTIVADOR P100, PROMUEVE LA SECRECION DE FOSFOLIPIDOS DE LIPOPROTEINAS EN HEPATOCITOS PRIMARIOS

Autores
Begoña Ochoa Olascoaga¹, Lourdes Palacios Martín², Olatz Fresnedo Aranguren³
1 Catedrática, Universidad del País Vasco, Lejona, España, Directora de Departamento
2 Investigadora y Profesora, Universidad del País Vasco, Lejona, España, Doctora
3 Profesora, Universidad del País Vasco, Lejona, España, Profesora Titular

Acceso a la fuente original
BBA Molecular and Cell Biology of Lipids
http://www.elsevier.com/wps/find/journaldescription.cws_home/506067/description

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